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100x lens is used with oil immersion
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∙ 13y ago
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Oil immersion is used as a medium between the specimen and the oil immersion objective lens why?
Oil immersion is a technique used to increase the resolution of a microscope.
Which objectives focuses closest to the slide?
oil immersion
What would occur if water were accidentally used in place of immersion oil?
When using the oil immersion objective, the oil has the same refractive index as the glass. So it is like an extension of the lens. Water does not have the same refraction index as glass, so the image would not be as clear.
Why should you never look at a wet mount slide with a oil immersion lens?
Wet mounts are not attached to the slide, it is "floating". If you use an oil immersion lens, you have to put a drop of oil on the slide cover so the light can correctly focus. The oil will cause the slide cover to stick to the end of your Oil immersion lens. When you focus the field of focus will stay the same since the slip cover is stuck to the lens. When you go to change objectives, the cover will travel with your oil immersion lense.
Why immersion oil use in gram staining?
because immersion oil has same refractive index as like lens glass of microscope so it give clear image of object
Oil immersion is used as a medium between the specimen and the oil immersion objective lens why?
Oil immersion is a technique used to increase the resolution of a microscope.
What oils can be used in oil immersion?
Cedar wood oil
Why do you use on or slide to be examined with the oil immersion objective?
If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.
Which objectives focuses closest to the slide?
oil immersion
What are the chemical called that are sometime used to make specimen visible?
immersion oil
Why isn't the oil immersion lens used in hanging drop procedure?
the hanging drop procedure uses a thicker slide, which could crack if the oil immersion lens is used. My bio professor worte: hanging drop slides asre too thick to use under oil.
What would occur if water were accidentally used in place of immersion oil?
When using the oil immersion objective, the oil has the same refractive index as the glass. So it is like an extension of the lens. Water does not have the same refraction index as glass, so the image would not be as clear.
What is the function of the oil used with the oil immersion lens?
So that you do not scratch the lens because at 100x magnification it is very very close to the slide.
When would you need to use the oil immersion lens?
Not only does oil immersion increase the microscopic resolution of a specimen, it is also transparent. This allows for optimal microscopy views.
How immersion oil helps in viewing an object?
This is because of the refractive propoties of the immersion oil. The oil works as a sort of secondary lens, magnifying the specimin in addition to the lens alresdy present.
What is the importance of oil in oil immersion lens of compound microscope?
In light microscopy, oil immersion is a technique used to increase the resolution of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens.
Why is 100x microscope lens called oil immersion lens?
Because that lens (it does not HAVE to be a 100x lens - other lens magnifications are also available in oil immersion constructions) is made to be in contact with a drop of special oil which covers the specimen. The oil has the same index of refraction as the front lens glass and that way a different optical construction can be used.
