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because immersion oil has same refractive index as like lens glass of microscope so it give clear image of object
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Why gram staining is classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
What is the advantage over the simple stain?
Gram staining highlights different bacteria types through the use of special dyes. It aids in the diagnosis of a specific organism and tells the difference between gram negative and gram positive bacteria. Simple staining is unable to highlight the exact organism.
Why should you never look at a wet mount slide with a oil immersion lens?
Wet mounts are not attached to the slide, it is "floating". If you use an oil immersion lens, you have to put a drop of oil on the slide cover so the light can correctly focus. The oil will cause the slide cover to stick to the end of your Oil immersion lens. When you focus the field of focus will stay the same since the slip cover is stuck to the lens. When you go to change objectives, the cover will travel with your oil immersion lense.
How do you remove oil from the slide and oil immerssion lens after use?
When you desire to remove oil from a slide and an immersion lens after using, for example, cedar oil, you would use Xylene to remove the oil. Natural oils can harden on the lens otherwise.
Which objective focuses closest to the slide when it is in focus?
The objective lens that focuses closest to the slide is 100x, it has the longest lens so the highest power. Be careful not to crack the slide and make sure you use oil if it is an oil immersion lens.
Why gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why is Gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why is gram staining important?
It tells the microbiologist/clinician facts about the cell wall construction of the bacterium. This then indicates which antibiotics to prescribe if you are trying to get rid of a bacterial infection.
Why do you use on or slide to be examined with the oil immersion objective?
If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.
Why gram staining is classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
What is the advantage of the gram stain over simple stains such as methylene blue?
Gram staining highlights different bacteria types through the use of special dyes. It aids in the diagnosis of a specific organism and tells the difference between gram negative and gram positive bacteria. Simple staining is unable to highlight the exact organism.
What is the advantage over the simple stain?
Gram staining highlights different bacteria types through the use of special dyes. It aids in the diagnosis of a specific organism and tells the difference between gram negative and gram positive bacteria. Simple staining is unable to highlight the exact organism.
Why should you never look at a wet mount slide with a oil immersion lens?
Wet mounts are not attached to the slide, it is "floating". If you use an oil immersion lens, you have to put a drop of oil on the slide cover so the light can correctly focus. The oil will cause the slide cover to stick to the end of your Oil immersion lens. When you focus the field of focus will stay the same since the slip cover is stuck to the lens. When you go to change objectives, the cover will travel with your oil immersion lense.
How do JCBs use hydraulics?
it runs with hydraulics .the hydraulics contains some oil immersion , fluids.
Why should the oil immersion objective be cleaned immediately after use?
Because the oil will dry and distort the refraction of the light through the objective.
How do you remove oil from the slide and oil immerssion lens after use?
When you desire to remove oil from a slide and an immersion lens after using, for example, cedar oil, you would use Xylene to remove the oil. Natural oils can harden on the lens otherwise.
Which objective focuses closest to the slide when it is in focus?
The objective lens that focuses closest to the slide is 100x, it has the longest lens so the highest power. Be careful not to crack the slide and make sure you use oil if it is an oil immersion lens.
