because immersion oil has same refractive index as like lens glass of microscope so it give clear image of object
The recommended type of immersion oil to use for microscopy is colorless, odorless, and has a refractive index closely matching that of glass, typically with a refractive index of 1.515.
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
Leaving immersion oil on the objective can attract dust and debris, which can impact imaging quality and potentially damage the lens. In addition, it can cause the oil to harden and become difficult to remove, leading to maintenance issues. Cleaning the oil off after use ensures optimal performance and prolongs the lifespan of the objective.
A 100x oil immersion objective is best for studying bacteria under a microscope as it provides the highest magnification and resolution needed to observe bacterial morphology and structure in detail. Additionally, the use of oil immersion helps to reduce the refraction of light and improve image clarity.
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
It tells the microbiologist/clinician facts about the cell wall construction of the bacterium. This then indicates which antibiotics to prescribe if you are trying to get rid of a bacterial infection.
If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.
The recommended type of immersion oil to use for microscopy is colorless, odorless, and has a refractive index closely matching that of glass, typically with a refractive index of 1.515.
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Gram staining highlights different bacteria types through the use of special dyes. It aids in the diagnosis of a specific organism and tells the difference between gram negative and gram positive bacteria. Simple staining is unable to highlight the exact organism.
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
A low power objective has a wider field of view and is easier to use because it doesn't require immersion oil. This makes it convenient for quickly scanning samples or observing larger structures on a slide without the need for oil immersion.
Leaving immersion oil on the objective can attract dust and debris, which can impact imaging quality and potentially damage the lens. In addition, it can cause the oil to harden and become difficult to remove, leading to maintenance issues. Cleaning the oil off after use ensures optimal performance and prolongs the lifespan of the objective.
In an oil immersion experiment, it's essential to ensure that the microscope lens and the slide are clean to prevent contamination and distortion of the image. Use only the recommended immersion oil to avoid damage to the optics. Additionally, handle the oil and glass slides carefully to avoid spills and breakage. Finally, after use, clean the lens and any spills immediately to maintain the equipment's integrity.
When viewing a slide under oil immersion, it is best to use the 100x objective lens. This high-power lens allows for greater magnification and resolution, making it ideal for observing fine details in the specimen. The use of immersion oil helps to reduce refraction of light and improves image clarity by providing a medium with a similar refractive index to glass.