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Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
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∙ 15y ago
Wiki User
∙ 9y agoGram staining can be used to classify bacteria into 2 specified groups; Gram positive such as Streptococcus and Staphylococcus and gram negative such as E.coli, Salmonella and Pseudomonas. Gram positive or negative is determined by the cell wall of a bacterium. It is a useful tool in determining bacteria along with other tests such as oxidase, catalase and other chemical reactions which for all effects narrow down the list until you find the family the bacteria is from then the species itself.
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What is the different staining technique in virology?
1- What_is_the_different_staining_technique_in_virology2- What are the diffrent stain in micro for virus ?
Which step is most crucial or most likely to cause poor results in the gram stain?
Gram staining is a type of differential staining in which two types of bacteria are differentiated on the basis of their cell wall either gram positive or gram negative although all the steps in gram staining are crucial, the most important step the most crucial step in the performance of the Gram staining procedure is the decolorization step which is the Acid-Alcohol (3% HCl and 95% Ethanol) and must be timed correctly; the crystal violet stain will be removed from both Gram-positive and negative cells if the decolorizing agent is left on too long (a matter of seconds).
A method called Gram staining is used to tell what?
Gram staining is primarily used to differential bacteria based on their cell wall structure. Bacteria are usually classified as Gram positive or Gram negative. Gram positive - thick layer of peptidoglycan as outermost layer, plasma membrane as innermost layer. Gram negative - thin layer of peptidoglycan "sandwiched" in between 2 separate plasma membranes.
A structural stain is considered to be a differential stain?
yup..two types of differential staining.. 1 - staining the whole cell to differentiate from another 2 - structural stainging of an individual cell
Is endospore staining differencial staining?
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
Why gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why is Gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
What is the different staining technique in virology?
1- What_is_the_different_staining_technique_in_virology2- What are the diffrent stain in micro for virus ?
What is the purpose of differential staining?
differential staining is a staining technique used to stain colorless bacteria against a dark background.
Grams method is used to tell what?
Gram staining is primarily used to differential bacteria based on their cell wall structure. Bacteria are usually classified as Gram positive or Gram negative. Gram positive - thick layer of peptidoglycan as outermost layer, plasma membrane as innermost layer. Gram negative - thin layer of peptidoglycan "sandwiched" in between 2 separate plasma membranes.
Which step is most crucial or most likely to cause poor results in the gram stain?
Gram staining is a type of differential staining in which two types of bacteria are differentiated on the basis of their cell wall either gram positive or gram negative although all the steps in gram staining are crucial, the most important step the most crucial step in the performance of the Gram staining procedure is the decolorization step which is the Acid-Alcohol (3% HCl and 95% Ethanol) and must be timed correctly; the crystal violet stain will be removed from both Gram-positive and negative cells if the decolorizing agent is left on too long (a matter of seconds).
Is gram staining related to flagella?
No. It is a staining on the cell itself.
Who developed Gram staining?
Gram staining was devised by Hans Christian Gram of Denmark in the 1800s. (1853-1938)
A method called Gram staining is used to tell what?
Gram staining is primarily used to differential bacteria based on their cell wall structure. Bacteria are usually classified as Gram positive or Gram negative. Gram positive - thick layer of peptidoglycan as outermost layer, plasma membrane as innermost layer. Gram negative - thin layer of peptidoglycan "sandwiched" in between 2 separate plasma membranes.
Why is gram staining important?
It tells the microbiologist/clinician facts about the cell wall construction of the bacterium. This then indicates which antibiotics to prescribe if you are trying to get rid of a bacterial infection.
A structural stain is considered to be a differential stain?
yup..two types of differential staining.. 1 - staining the whole cell to differentiate from another 2 - structural stainging of an individual cell
Why is safrinin used in gram staining?
as a couterstain
