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Although glycogen is a carbohydrate, it is classified under POLYSACCHARIDES which are non-reducing sugars. Benedict's reagent is used to test for the presence of non-reducing sugars. The positive result for this is a brick-red precipitate or solution. It remained blue (which is negative) due to fact that ALL POLYSACCHARIDES (such as glycogen) ARE NON-REDUCING SUGARS
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Why is sodium carbonate added to solution before Benedicts test is performed?
Sodium carbonate is added to increase the pH of the solution.
Why Na2CO3 is added to solution before Benedicts test is performed?
Na2CO3 is added to the solution before the Benedict's test to create an alkaline environment, which helps to stabilize the blue copper (II) ions in the Benedict's reagent. This stabilization is important for the detection of reducing sugars, as the copper (II) ions must be reduced to form a red precipitate in the presence of reducing sugars.
Why Na2Co3 is added with CuSo3 at preparing benedict's reagent?
just for FUN!
What is a excess reagent?
In most reactions there are two types of reactant. One of them is completely consumed and the other is only partially consumed. The reactant that is only partially consumed is the excess reactant.
What chemical reagent of protein?
Biuret reagent is used to test for protein in urine. It is a common test that students in biology class perform. Urine is added to a test tube, followed by approximately the same amount of Biuret reagent. If the solution turns lavender this means that there are proteins present in the urine.
Why do you heat substanses with Benedict's reagent with them while testing?
Answer:- It requires energy for the Cu2+ ions in the Benedicts to be reduced to Cu+ Explanation:- Benedicts reagent indicates a reducing sugar because it consists of copper (II) sulphate in an alkaline solution. Its distinctive blue colour turns brick red when it is added to a reducing agent because the Cu2+ ions will be reduced, resulting in copper (I) sulphate, which is brick red. This is where the term "reducing sugars" comes from. All sugars that reduce the benedicts have an available aldehyde or ketone group. It is this group that provides the electron needed to reduce the Benedict's. Because of ionisation energies, even when the reducing sugar is present energy is required to remove the electron from the valence shell. This is why heat is required. It does not, as the previous answer stated, have anything to do with enzymes. Enzymes are globular protein molecules whereas saccharides are ring structured carbohydrate molecules. Do not use any of that answer in any work you might do!
What colour does egg white turn when millon's reagent is added?
When Millon's reagent is added to egg white, it typically turns a pink or red color. This reaction occurs because Millon's reagent is used to detect phenolic compounds, including tyrosine, which is present in proteins like those found in egg white. The formation of this color indicates the presence of these amino acids in the sample.
What is the process of turning glucose to glycogen?
Glucose is converted to glycogen through a process called glycogenesis. In this process, glucose molecules are added to a growing glycogen chain by the enzyme glycogen synthase, utilizing UDP-glucose as a substrate. This conversion primarily occurs in the liver and muscles to store excess glucose for later use as an energy source.
What is the significance of the nonreducing end of glycogen in terms of its structure and function?
The nonreducing end of glycogen is important because it is where new glucose units are added during glycogen synthesis. This end of the molecule is not involved in reducing sugars and plays a key role in the branching structure of glycogen, allowing for efficient storage and quick release of glucose when needed for energy.
What is Back titration?
A back titration is a form of titraiton in which an excess of standard reagent is added and then the reverse of the titration is carried out.
Why does glycogen change to red when iodine solution is added?
Iodine tests for complex sugars. Glycogen is a complex sugar and will change dark purple when iodine solution is added (color is irrelevant, all you really need to know is that the change to a specific color signifies a presence of a macro molecule)
What is borsch reagent?
Borsch reagent is a chemical solution commonly used in microbiology to detect the presence of indole. It is composed of hydrochloric acid, dimethylaminobenzaldehyde, and amyl alcohol. When added to a bacterial culture, the reagent reacts with indole produced by certain bacteria, resulting in a color change from yellow to red.
