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Let's say the objects are initially neutral, and somehow the charges are separated, so that one remains with a positive charge, and the other, with a negative charge. I am assuming that this is, more or less, what you mean.

What happens here is that charged particles - often electrons, but it can be other charged particles as well - are transferred from one object to the other. Let's assume it is electrons. The object to which the electrons move will have more negative charges; thus, it will end up with a net negative charge. The other object will remain with a net positive charge, since it has the same number of positive charges than before, but less negative charges.

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