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Because if you use the coarse adjustment under high power, there is a greater risk that you will crack the slide on the microscope and damage it. The fine adjustment only moves the objective lense small amounts and very slowly, so there is no chance that you will damage the slide.
The low power objective is used first for two reasons: First, focusing the low power objective will insure that the other objectives will be at the proper height. Smashing a slide with an objective can damage the objective. Second, you can see a greater area with the low powered objective. This allows you to easily locate an object of interest, center it in frame and then zoom in on it.
The low power objective is useful for allowing you to focus your lens on the object that you wish to study without too much difficulty. Then it should be possible to simply switch to a higher power objective lens without having to do more than make a small adjustment to the fine focus knob. Another advantage of the lower power lenses is to allow scanning for something that may be of interest, without needing to examine each field in minute detail when the organism/organelle of interest is not present. When you see something of potential interest, you can then switch to a higher power lens.
slide? your obviously doing something for cyberschool and you need to use the slide viewer =P ^ Retard... Slide referring to the slide under the microscope. And the answer would be the inner and outermembrane.
This would depend on the type of slide and type of microscope; however, typically you turn on your light source (either electric or sometimes a mirror to reflect the ambient light), switch your magnification to the lowest power (usually scanning at 4X) place the slide on the stand and hold it with two clips, adjust the height of the stand with the gross adjustment knob (larger) until the image comes into view, then use the fine adjustment knob to focus the image. You can then change magnification power as high as your microscope will go. Note: If your microscope has a 1000X oil immersion setting, make sure to follow the instructions for oil immersion or you can damage your lense. Also, once you get past the 10X, only use the fine adjustment knob or you can damage the lense.
10x
start on the lowest objective
10x
40x
50x
The lowest power objective lens should be under the tube. This provides clear access, prevents accidents, and lets you locate the object and reposition the slide as needed.
Place slide on stage and stabilize w/clipsCenter slide on stageLowest-power objective lens is in placeDecrease between stage and tip of objective lensFocus with coarse and fine knobs
it has to do with the resolution of the image of the slide obtained...the larger the objective lens value is the greater the resolution it will provide, greater the resolution higher the details.
Place slide on stage and stabilize w/clipsCenter slide on stageLowest-power objective lens is in placeDecrease between stage and tip of objective lensFocus with coarse and fine knobs
Actually, it depends on the specimen which you are observing.. Suppose, if you are observing Tissues then you have to switch to 10X objective; but if you are observing mammalian cells or other cellular specimen then you have to switch to 45X objective...But, if you want to observe microbial cells then you have to put a drop a drop of paraffin oil on the smear made on the slide and then observe it into 100X oil immersion objectivelens...
You don't want yucky stuff mushing itself onto your objective lens. Slide covers save you from constantly cleaning your lens.
When using a microscope, the initial focusing of any slide is done under either the 4x or 10x objective. Once you have your subject in focus, you may make further adjustments.