0
Want this question answered?
Be notified when an answer is posted
Add your answer:
Earn +20 pts
What was the purpose of examining the original pGLO solution with and without UV illumination?
The purpose of examining the original pGLO solution with UV illumination was to detect bacteria. The purpose of examining the original pGLO solution without UV illumination was to detect the possibility that DNA cells existed.
Which plates should be compared to prove that the changes that occurred were due to the procedure that you performed?
The best way to prove that these changes occurred in the transformation lab is to compare the control to the experimental plates. Cells that were not treated with the plasmit (LB/amp (-) pGLO and LB/amp/are (-) pGLO plates) could not grow on ampicillin, wheras cells that were treated with the plasmid (LB/amp (=) pGLO and lB/amp/ara (+) pGLO plate) can grow on the LB/amp plate. Thus, the plasmid must confer resistance to ampicillin.
Where the gene come from?
read the book
Where is the milano gene located and how different isit from a normal gene?
all of your gene come from geniration and start with your longevity digest
Where do proper enzymes come from?
synthesized from coded information of gene
What was the purpose of examining the original pGLO solution with and without UV illumination?
The purpose of examining the original pGLO solution with UV illumination was to detect bacteria. The purpose of examining the original pGLO solution without UV illumination was to detect the possibility that DNA cells existed.
How do ampicillin resistant E. coli combat the lethal effects of the ampicillin antibiotic?
The pGlo plasmid contains an ampicillin resistance gene. This gene encodes an enzyme, β lactimase, which enzymatically degrades ampicillin. Therefore, bacteria that take up the plasmid (transformants) become resistant to ampicillin.
Why use arabinose in the plate in pglo experiments?
The arabinose sugar binds to the RNA in the cell and helps turn on the gene expression to make GFP, green flourescent proteins. When you shine a UV light on the proteins, it will glow.
Which plates should be compared to prove that the changes that occurred were due to the procedure that you performed?
The best way to prove that these changes occurred in the transformation lab is to compare the control to the experimental plates. Cells that were not treated with the plasmit (LB/amp (-) pGLO and LB/amp/are (-) pGLO plates) could not grow on ampicillin, wheras cells that were treated with the plasmid (LB/amp (=) pGLO and lB/amp/ara (+) pGLO plate) can grow on the LB/amp plate. Thus, the plasmid must confer resistance to ampicillin.
What traits of the E. coli were not altered in pGLO experiment?
Colony shape and color
What is gene doping?
Gene doping is when you take a gene from your body and keep it to mix with another gene, so that the out come is a perfect sportsman.
What are the two possible sources of fluorescence within an Ecol colony when exposed to UV light?
pGLO and GFP
Where the gene come from?
read the book
Where is the milano gene located and how different isit from a normal gene?
all of your gene come from geniration and start with your longevity digest
How can both your parents have brown eyes and you have blue?
It means they carried the gene for blue eyes as a recessive gene. If they both gave you this gene, then you will come out with blue eyes.
What is delayed-action gene?
A gene that takes time to come into effect such as puberty hormones and greying hair.
Where do proper enzymes come from?
synthesized from coded information of gene
