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What else can I help you with?
What was the purpose of examining the original pGLO solution with and without UV illumination?
The purpose of examining the original pGLO solution with UV illumination was to detect bacteria. The purpose of examining the original pGLO solution without UV illumination was to detect the possibility that DNA cells existed.
Which plates should be compared to prove that the changes that occurred were due to the procedure that you performed?
The best way to prove that these changes occurred in the transformation lab is to compare the control to the experimental plates. Cells that were not treated with the plasmit (LB/amp (-) pGLO and LB/amp/are (-) pGLO plates) could not grow on ampicillin, wheras cells that were treated with the plasmid (LB/amp (=) pGLO and lB/amp/ara (+) pGLO plate) can grow on the LB/amp plate. Thus, the plasmid must confer resistance to ampicillin.
Where the gene come from?
read the book
Where do proper enzymes come from?
synthesized from coded information of gene
Chromosomes come in pairs known as what?
Homologous chromosomes
What was the purpose of examining the original pGLO solution with and without UV illumination?
The purpose of examining the original pGLO solution with UV illumination was to detect bacteria. The purpose of examining the original pGLO solution without UV illumination was to detect the possibility that DNA cells existed.
Why use arabinose in the plate in pglo experiments?
Arabinose is used in the plate in pglo experiments to induce the expression of the Green Fluorescent Protein (GFP) gene. The presence of arabinose activates the araC promoter, allowing for the transcription and translation of the GFP gene, which results in the production of green fluorescent protein in the bacteria. This fluorescence helps researchers visualize and track the transformation of the bacteria with the desired gene.
What is meant by a control plate in pglo experiments?
a control plate in your particular case is a ampicillin plate with no inoculation of your transformed bacteria. its purpose to make sure that during the process of handling the plate there were no contamination by bacteria in the surrounding.
How do ampicillin resistant E. coli combat the lethal effects of the ampicillin antibiotic?
The pGlo plasmid contains an ampicillin resistance gene. This gene encodes an enzyme, β lactimase, which enzymatically degrades ampicillin. Therefore, bacteria that take up the plasmid (transformants) become resistant to ampicillin.
Which plates should be compared to prove that the changes that occurred were due to the procedure that you performed?
The best way to prove that these changes occurred in the transformation lab is to compare the control to the experimental plates. Cells that were not treated with the plasmit (LB/amp (-) pGLO and LB/amp/are (-) pGLO plates) could not grow on ampicillin, wheras cells that were treated with the plasmid (LB/amp (=) pGLO and lB/amp/ara (+) pGLO plate) can grow on the LB/amp plate. Thus, the plasmid must confer resistance to ampicillin.
What traits of the E. coli were not altered in pGLO experiment?
Colony shape and color
What is gene doping?
Gene doping is when you take a gene from your body and keep it to mix with another gene, so that the out come is a perfect sportsman.
Where the gene come from?
read the book
Where does new DNA come from in gene cloning?
New DNA molecules can come from various sources in gene cloning, such as PCR amplification of a specific gene, synthesis of a gene using recombinant DNA technology, or isolation of a gene from a donor organism. These DNA molecules are then inserted into a vector, such as a plasmid, to create a recombinant DNA molecule for cloning.
How can both your parents have brown eyes and you have blue?
It means they carried the gene for blue eyes as a recessive gene. If they both gave you this gene, then you will come out with blue eyes.
Where do proper enzymes come from?
synthesized from coded information of gene
Bio ap ap pglo transformation?
What kind of question is that? I mean really genius. I mean all your saying is the labs name and the class...that is not a question.
