LIVER
hypotonic solution
it's already answered in yahoo answer. just type "why does hemolysis occur when red blood cells" :D not too long. i was looking for the answer too and found it:D
When red blood cells draw in water and burst, this process is called hemolysis. Hemolysis can occur due to various factors such as osmotic imbalances, toxins, or physical damage to the cell membrane.
Alpha hemolysis is partial hemolysis resulting in a greenish discoloration of the agar, beta hemolysis is complete hemolysis resulting in a clear zone around the colony, and gamma hemolysis is no hemolysis observed.
While the spleen does play a role in the removal and recycling of old or damaged red blood cells, it is not the primary site of hemolysis. Hemolysis, the breakdown of red blood cells, can occur in various parts of the body, including in the bloodstream, liver, and bone marrow.
The three types of hemolysis are alpha hemolysis (incomplete hemolysis, causing a greenish discoloration around bacterial colonies), beta hemolysis (complete hemolysis, causing a clear zone around bacterial colonies), and gamma hemolysis (no hemolysis, with no change in the appearance of blood agar).
Hemolysis
The hemolysis is called green hemolysis because of the color change in the agar.
Hemolysis typically occurs in red blood cells when they are exposed to a NaCl concentration below approximately 0.45% (or 0.15 M). At this concentration, the osmotic pressure causes water to enter the cells, leading to swelling and eventual rupture. Conversely, NaCl concentrations above this level are generally isotonic or hypertonic, preventing hemolysis.
Hemolysis is produced as a result of changes in osmotic pressure, leading to the rupture of red blood cells and the release of hemoglobin into the surrounding fluid. This can occur when red blood cells are exposed to hypotonic solutions causing them to swell and burst.
Target hemolysis is caused by the bacteria closteridium perfringens. there is a narrow zone of complete hemolysis due to theta toxin sorrounded by incomplete zone of hemolysis due to alpha toxin
Micrococcus luteus typically displays gamma hemolysis on a blood agar plate, which means it does not cause any hemolysis of the red blood cells.