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How is plasmid DNA inserted into the cell where the DNA originally come from?
by transformation
Why the cell do not contain plasmid if they not grow in antibiotics?
If you do not give antibiotics, the plasmid may lost after a few generation. it is because bacteria thinks that the extra DNA (plasmid) is not needed as the antibiotic is not present. That is the reason for keeping the culture always in antibiotic selection pressure.
A bacterial cell that has taken up plasmid DNA is?
called a transformed bacterial cell. The plasmid DNA can confer specific traits to the bacterial cell, such as antibiotic resistance, ability to produce certain proteins or enzymes, or other desired characteristics. This process is commonly used in genetic engineering and biotechnology research.
How can bacteria be transformed with recombinant plasmid?
Bacteria can be transformed with recombinant plasmid by introducing the plasmid into the bacterial cell through a process called transformation. This allows the bacteria to take up the recombinant DNA from the plasmid and express the desired gene or trait encoded in the DNA.
Why T-DNA is not degraded by plant cell?
T-DNA, which is part of the Ti plasmid, is protected by vir proteins (encoded by vir genes also present on the Ti plasmid) after it is excised. Plant restriction enzymes cannot attack T-DNA bound my vir proteins since the enzyme cannot gain access to the interiors of the DNA.
Do plasmid act as a carrier of foreign DNA and is needed to clone a gene?
The host cell will have a repairing mechanism after the introduction of foreign DNA. In order to reduce the chances of the insert plasmid to be repaired and removed, amount of carrier DNA (foreign DNA) are added. By removing the carrier DNA, inserted plasmid will have great opportunity to integrate with the host cell after repairing.
How is plasmid DNA similar to the DNA found in chromosomes?
Plasmid DNA is similar to chromosomal DNA in that it is double-stranded and carries genetic information in the form of genes. Both types of DNA can replicate and be passed on to new cells during cell division. However, plasmid DNA is typically smaller and found in the cytoplasm of the cell, separate from the chromosomal DNA located in the nucleus.
What is the origin of replication in an expression plasmid?
The origin of replication in an expression plasmid is a specific DNA sequence that allows the plasmid to replicate, or make copies of itself, within a host cell. This sequence is essential for the plasmid to be maintained and passed on to daughter cells during cell division.
What does transformation and competent and plasmid mean?
transformation is to introduce a foreign DNA segment to a competent cell. competent cell means a cell that have ability to take a foreign DNA. plasmid is genetic element that one can integrate a DNA segment. it has its promoter, resistance gene, multiple cloning site...
What is an extrachromosomal piece of bacterial DNA that contains genes not essential for cell growth?
A Plasmid
What do you call a population of cells carrying a desired plasmid?
A plasmid is a small molecule of DNA that replicate independently within the cell. A population of cells carrying a desired plasmid is called a clone.
What is mode of action of acridine orange in plasmid curing?
Acridine orange can intercalate with DNA and induce frameshift mutations, leading to DNA damage and subsequent cell death. In plasmid curing, this can result in the loss of plasmids from bacterial cells, as the damaged DNA is eliminated during replication or cell division.
