pyruvate carboxylase and phosphoenolpyruvate carboxykinase (PEPCK)
Examples of enzymes used in glycolysis include hexokinase, phosphofructokinase, and pyruvate kinase. These enzymes play key roles in catalyzing specific reactions within the glycolytic pathway, helping to break down glucose into pyruvate and generate ATP.
Glucokinase, phosphofructokinase-1, pyruvate kinase
Glycolysis primarily occurs in the cytoplasm of the cell. It involves various enzymes such as hexokinase, phosphofructokinase, and pyruvate kinase. Glycolysis also requires substrates like glucose and ATP, and produces molecules such as pyruvate, ATP, and NADH.
Hexokinase
The irreversible reactions in glycolysis are catalyzed by the enzymes hexokinase, phosphofructokinase, and pyruvate kinase. These reactions involve the conversion of glucose to glucose-6-phosphate, fructose-6-phosphate to fructose-1,6-bisphosphate, and phosphoenolpyruvate to pyruvate, respectively.
The reactions of glycolysis occur in the cytoplasm of a eukaryotic cell. The enzymes required for glycolysis are found in the cytoplasm, where glucose is broken down into pyruvate to produce energy in the form of ATP.
In humans, pyruvate cannot be directly converted into glucose through a process called gluconeogenesis. This is because humans lack the specific enzymes required to convert pyruvate into glucose. Pyruvate can be converted into lactate or acetyl-CoA, which can then enter various metabolic pathways in the body.
Pyruvate enters the Krebs cycle via an intermediate called acetyl CoA.Pyruvate is formed in the cytoplasm as the end product of glycolysis. Pyruvate enters a mitochondrion, in the matrix of which it encounters the enzyme pyruvate dehydrogenase. This enzyme catalyzes the reaction of pyruvate with coenzyme A to form acetyl CoA. This reaction is sometimes called the link reaction, as it links glycolysis to the Krebs cycle (= citric acid cycle, or tricarboxylic acid cycle)."Pyruvate dehydrogenase" is in fact a huge complex (bigger than a ribosome) consisting of three enzymes and a number of other substances, including coenzymes. In the course of the reaction NAD+ is reduced to NADH. A molecule of CO2 is also produced. So this reaction involves a both an oxidation and a decarboxylation.Acetyl CoA then reacts with oxaloacetate to form citrate. Both oxaloacetate and citrate are intermediates of the Krebs cycle.
Magnesium is not a direct cofactor of glycolysis, but it is required for the activity of some enzymes involved in glycolysis. For example, magnesium is needed for the activation of the enzyme phosphofructokinase, which is a key regulatory enzyme in glycolysis. Magnesium helps stabilize the ATP molecule during the phosphorylation reactions in glycolysis.
The irreversible reactions of glycolysis are catalyzed by enzymes hexokinase, phosphofructokinase, and pyruvate kinase. These reactions help regulate the flow of glucose through the pathway by committing glucose to be broken down into pyruvate. This regulation ensures that glycolysis proceeds efficiently and that the cell can generate energy effectively.
Substrates are the products of enzyme activity when metabolism occurs. For example: with glycolysis hydrogen is a substrate that is carried to the electron transport chain by NAD and FAD which then become NADH and FADH with the added hydrogen. I am just a student though, i was looking for the same answer. Just know what is produced as a result of a particular reaction and you will have your substrates. Shane
There are 1000's of enzymes in the body. In a biochemical pathway there maybe be multiple enzymes used! for examply, glycolysis..