The cloning capacity of pBR322 vector is 1-5kb.
i have no idea wat plasmid,cloning ,and vector means so haha 2 u
The good cloning vector should have small size, high copy number, own origin of replication, restriction sites for many restriction enzymes and selectable markers.
who invented (discovered) cloning?Cloning technology began with the work of Hans Adolf Edward Driesch. In 1885, he was able to demonstrate the first artificial embryo twinning of sea urchins.
No, not really since it is just for cloning. But their should be enough promoter/sequence to provide antibiotic resistance.
The cloning capacity of pBR322 vector is 1-5kb.
i have no idea wat plasmid,cloning ,and vector means so haha 2 u
plasmid is the type of the cloning vector. other cloning vectors includes cosmids, bacteriophage, phagemids, artifiical chromosomes. clonong vectors are the carriers of certain traits to be inserted in non coding regions of the DNA.
Gene Cloning is used to clone a gene of interest in a vector called plasmid. The chimeric DNA or rDNA formed by cloning is stable and can be used to propagate and sequence the DNA. producing vector containing inulin gene is an example.
The insert capacity of a cosmid vector is about 35-45 kb.
The good cloning vector should have small size, high copy number, own origin of replication, restriction sites for many restriction enzymes and selectable markers.
Expression vectors contain translation start and termination codons. Moreover, it contains promoter region
who invented (discovered) cloning?Cloning technology began with the work of Hans Adolf Edward Driesch. In 1885, he was able to demonstrate the first artificial embryo twinning of sea urchins.
Competent cells if i not mistaken
No, not really since it is just for cloning. But their should be enough promoter/sequence to provide antibiotic resistance.
there are two types of vectors cloning vector and expression vectors.
We can insert about 5-25 kb sized foreign DNA in a lambda bacteriophage vector.