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The fragment of the DNA that is the longest is the one that is the slowest to get to the bottom of the gel in the body. This is because longer DNA are simply the largest base pairs that are digested in the restriction enzymes which make them slower then the shorter ones.

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Q: Which fragment of DNA is the longest or biggest?
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Related questions

Why is the largest DNA fragment band found closest to the well in which it was placed?

The largest DNA fragments travel more slowly through the agarose gel due to their size, so they don't move as far from the well as smaller fragments during gel electrophoresis. This results in the largest fragments being closest to the well after electrophoresis is completed.


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Why does the DNA fragment size change as you move from the cathode to the anode?

In gel electrophoresis, DNA fragments move towards the anode (positive electrode) because DNA is negatively charged. Smaller fragments move faster through the gel matrix, so they appear closer to the anode while larger fragments move slower and appear closer to the cathode. This results in separation of DNA fragments based on size.


What type of enzyme is used to fragment dna?

Restriction enzymes are used to fragment DNA by cutting it at specific recognition sites. These enzymes are naturally found in bacteria as a defense mechanism against foreign DNA, and are commonly used in molecular biology techniques like restriction enzyme digestion.


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DNA ligase plays a crucial role in the elongation of the lagging strand during DNA replication by sealing the nicks between the Okazaki fragments. It catalyzes the formation of phosphodiester bonds between adjacent nucleotides, joining the fragments together to form a continuous strand. This ensures the completion of the synthesis of the lagging strand and the formation of a fully functional double-stranded DNA molecule.


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