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"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".
Commonly used vectors include plasmid, Lambda phage, cosmid and yeast artificial chromosome (YAC).
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What are temperate phages?
A phage that can enter into lysogeny with its host. A phage that can become a prophage.
When a phage attaches to a bacterium what does the phage inject and what stays attached to the cell?
The part that remains outside is called the capsid or protein coat.
What is the difference between a virulent phage and a temperate phage?
In virology, temperate refers to the life cycle some phages are able to perform. Atemperate phage can integrate its genome into its host bacterium's chromosome, becoming a lysogen known as a prophage. A temperate phage is also able to undergo lytic life cycles, where the prophage is expressed, replicates the phage genome and produces phage progeny and the progeny phage leave the bacterium. The virulent phages have only lytic lifecycles and thus infection results in the host cell's death (due to lytic cell destruction-the phage replicates itself and then bursts the cell, releasing many copies).
What does phage DNA do once inside the host cell?
Once inside the host cell, phage DNA utilizes the host cell's machinery to replicate itself. It takes over the cellular processes and redirects them towards the production of new phages. The phage DNA is transcribed and translated to synthesize the necessary components for phage assembly, ultimately resulting in the formation of progeny phages inside the host cell.
What is a virus that infects E coli bacteria?
T4 Phage
What does a phage inject when it attaches to a bacterium?
Dna and phage body
What is the need for phage titration?
PHAGE TRTRATION IS DONE SO AS TO FIND THE AMOUNT OF PHAGE PARTICLES PRESENT IN THE STOCK PHAGE TRTRATION IS DONE SO AS TO FIND THE AMOUNT OF PHAGE PARTICLES PRESENT IN THE STOCK
What is another term for a lysogenic phage?
temperate phage
A virus that reproduces in a bacterium is called?
phage
When phage attaches to a bacteria the phage injects what?
DNA single
What is temperate phage superinfection immunity?
c. Repression of the phage genome - A phage coded protein, called a repressor, is made which binds to a particular site on the phage DNA, called the operator, and shuts off transcription of most phage genes EXCEPT the repressor gene. The result is a stable repressed phage genome which is integrated into the host chromosome. Each temperate phage will only repress its own DNA and not that from other phage, so that repression is very specific (immunity to superinfection with the same phage).Reference: http://pathmicro.med.sc.edu/mayer/phage.htm
What are temperate phages?
A phage that can enter into lysogeny with its host. A phage that can become a prophage.
When a phage attaches to a bacterium what does the phage inject and what stays attached to the cell?
The part that remains outside is called the capsid or protein coat.
What is SDS phage?
there is nothing like SDS phage but... 1. SDS is a well know detergent used to denature proteins before electrophoresis called SDSPAGE. 2. phage (bacteriophage) is a virus that infects the bacteria which contains eother DNA or RNA. SDS PAGE can be used to determine the phage proteins which u can call SDSPAGE of phage.
What actors and actresses appeared in Phage - 1997?
The cast of Phage - 1997 includes: Jasper Bagg as Burke
When the bacteriophage DNA becomes part of the bacterial chromosome?
During phage infection into bacteria, it penetrates phage DNA into bacterium,which will be integrated in to the bacterial genome (chromosome) to replicate and synthesize phage molecules.
When a T2 bacteriophage infects an E. coli cell what part of the phage enters the bacterial cytoplasm?
the whole phage
