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What is the difference between cosmid and plasmid?
A cosmid is a type of hybrid vector that combines features of a plasmid and a bacteriophage. It can carry larger DNA fragments (up to 45kb) compared to plasmids (up to 10kb). Cosmids have a plasmid backbone for replication in bacteria and a phage attachment site for packaging DNA into phage particles for transfer into host cells.
What are phage vectors?
"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".Commonly used vectors include plasmid, Lambda phage, cosmid and yeast artificial chromosome (YAC).
Why bacillus anthracis susceptible to gamma phage?
Bacillus anthracis is susceptible to gamma phage because the phage has evolved to target and infect specific receptors on the surface of the bacterium. This interaction enables the gamma phage to inject its genetic material into the bacterium, hijacking its machinery to produce more phage particles and ultimately leading to the destruction of the bacterial cell.
When a phage attaches to a bacterium what does the phage inject and what stays attached to the cell?
When a phage attaches to a bacterium, it injects its genetic material (DNA or RNA) into the cell. The phage capsid (outer protein coat) typically stays attached to the cell surface during this process.
How do restriction enzymes create recombinant DNA?
There are many methods, though one of the most common is the use of restriction endonucleases. These enzymes can be used to cut DNA fragments at specific locations. Cut DNA fragments will recombine into new orders, which are sealed using DNA ligase. A selection process must be used to locate the desired recombinant DNA, since it will be in a mixture of various undesired recombinations.
Lamda phage of E. coli?
It is the 'T4phage' or 'coliphage lambda'.
What is the difference between cosmid and plasmid?
A cosmid is a type of hybrid vector that combines features of a plasmid and a bacteriophage. It can carry larger DNA fragments (up to 45kb) compared to plasmids (up to 10kb). Cosmids have a plasmid backbone for replication in bacteria and a phage attachment site for packaging DNA into phage particles for transfer into host cells.
What are phage vectors?
"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".Commonly used vectors include plasmid, Lambda phage, cosmid and yeast artificial chromosome (YAC).
What are two types of vector used in recombinant DNA technology?
there are many different vectores as: 1-plasmid system 2-bacteria phage lamda 3-cosmids 4-bacterio artificial system 5-puc system the other cloning vectors are m13 which is the oldest one. and after the above all are:- BAC(bacterial artificial chromosome) YAC(yeat artificial chromosome) TAC(transformation-competent artificial chromosome)
What is the insert capacity of a P1 phage in DNA cloning?
You can add maximum 70-100 kb of genetic material in a P1 phage vector.
How is a genomic library produced?
A genomic library is produced by isolating DNA from an organism, fragmenting it into smaller pieces, and inserting these fragments into a vector (such as a plasmid or a phage). The vector is then introduced into a host organism, such as bacteria, which will replicate the DNA fragments along with their own DNA, creating a library of the organism's entire genome.
Is mcs a cloning vector?
MCS (Multiple Cloning Site) is not a cloning vector itself, but rather a region within a vector that contains multiple restriction sites for inserting DNA fragments during the cloning process. Common vectors that contain an MCS include plasmids and phage vectors.
A virus that reproduces in a bacterium is called?
phage
What is another term for a lysogenic phage?
temperate phage
When phage attaches to a bacteria the phage injects what?
DNA single
The substance a phage leaves outside its host cell?
The substance a phage leaves outside its host cell is typically referred to as the "phage progeny" or "phage particles." These particles can include newly synthesized phage DNA and proteins, as well as the phage capsid that encapsulates the genetic material. This material can go on to infect other host cells and continue the phage replication cycle.
What is the need for phage titration?
Phage titration is needed to determine the concentration or titer of phages in a sample, which is crucial for ensuring the effectiveness of phage-based therapies, determining the optimal phage concentration for experiments, and monitoring phage growth dynamics in culture. Titration allows researchers to quantify the number of active phages present in a sample, providing valuable information for various applications in phage research and biotechnology.
