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What is the insert capacity of a cosmid vector in DNA cloning?
The insert capacity of a cosmid vector is about 35-45 kb.
What is the difference between plasmid and episomes?
Plasmid DNA is an extra-chromosomal DNA molecule, it cannot link up with chromosomal DNA, and it contains the genetic informations that are necessary for its own replication. Episomes is any kind of extra-chromosomal DNA that can link up with chromosomal DNA. That is the main difference between them two. Episomes are usually larger than other extra-chromosomal DNA. An example of episome are the viruses, because they intergrate their genetic material into the host's chromosomal DNA.The only difference between the plasmid and the episome is the integration i-e plasmid can not integrate while episome can integrate into the genome.
What is the difference between the original plasmid and the recombinant plasmid?
Orginal Plasmids are extra chromosomal genetic material present in eukaryotes and some prokaryotes.Recombinant plasmids contain a gene of intrest ie,individual gene carrying a specific function can be inserted in to a specific site on original plasmid in cell culture via transformation.So the recombinant plasmid contain both gene of intrest and native genes.
What are small circular segments of DNA often exchanged between bacteria?
Plasmid
The Ti plasmid from which plant pathogen is used to transfer plant genes between species?
The Ti plasmid is derived from Agrobacterium tumefaciens, which is a plant pathogen. This plasmid is commonly used as a vector to transfer foreign genes into plant cells in genetic engineering applications.
What are some of the characteristics of cosmid?
A cosmid has the characteristics of a hybrid plasmid. They are used in the building of genomic libraries. They are ~200 base pairs long. More detailed information is available on Wikipedia.
What is a small double stranded DNA that's used as a vector?
it is a ds DNA use in recombinant DNA technology to insert our interested gene and multiply it.Ex;plasmid,cosmid
What is the insert capacity of a cosmid vector in DNA cloning?
The insert capacity of a cosmid vector is about 35-45 kb.
What is the difference between recombination and transformation?
Recombinants take up the plasmid but do not incorporate it into the DNA. Transformants take up the plasmid and integrate it into the DNA. Recombinants wouldn't express the new genes while Transformants will.
What is the difference between plasmid and episomes?
Plasmid DNA is an extra-chromosomal DNA molecule, it cannot link up with chromosomal DNA, and it contains the genetic informations that are necessary for its own replication. Episomes is any kind of extra-chromosomal DNA that can link up with chromosomal DNA. That is the main difference between them two. Episomes are usually larger than other extra-chromosomal DNA. An example of episome are the viruses, because they intergrate their genetic material into the host's chromosomal DNA.The only difference between the plasmid and the episome is the integration i-e plasmid can not integrate while episome can integrate into the genome.
What is the difference between the original plasmid and the recombinant plasmid?
Orginal Plasmids are extra chromosomal genetic material present in eukaryotes and some prokaryotes.Recombinant plasmids contain a gene of intrest ie,individual gene carrying a specific function can be inserted in to a specific site on original plasmid in cell culture via transformation.So the recombinant plasmid contain both gene of intrest and native genes.
What are small circular segments of DNA often exchanged between bacteria?
Plasmid
How you clone a gene using cosmid as a vector?
The first step is restriction of the cosmid and the foreign DNA with the restriction enzyme, then ligating the fragments together. Thereafter, the cosmids are loaded into the phage capsid, which leads to the expression of the foreign gene through transduction.
Which is the plasmid that increases resistance to antibiotics?
R-plasmid
What plasmid that can degrade toluic acid?
TOL plasmid
Genes that encode for drug resistance and can be shared between bacteria are often found on?
plasmid
The Ti plasmid from which plant pathogen is used to transfer plant genes between species?
The Ti plasmid is derived from Agrobacterium tumefaciens, which is a plant pathogen. This plasmid is commonly used as a vector to transfer foreign genes into plant cells in genetic engineering applications.
