cosmids can accept insert fragments of DNA from 30-50 kilobases but plasmids can only accept inserts that are up to 15kb only.
The insert capacity of a cosmid vector is about 35-45 kb.
Plasmid DNA is an extra-chromosomal DNA molecule, it cannot link up with chromosomal DNA, and it contains the genetic informations that are necessary for its own replication. Episomes is any kind of extra-chromosomal DNA that can link up with chromosomal DNA. That is the main difference between them two. Episomes are usually larger than other extra-chromosomal DNA. An example of episome are the viruses, because they intergrate their genetic material into the host's chromosomal DNA.The only difference between the plasmid and the episome is the integration i-e plasmid can not integrate while episome can integrate into the genome.
Orginal Plasmids are extra chromosomal genetic material present in eukaryotes and some prokaryotes.Recombinant plasmids contain a gene of intrest ie,individual gene carrying a specific function can be inserted in to a specific site on original plasmid in cell culture via transformation.So the recombinant plasmid contain both gene of intrest and native genes.
Plasmid
The Ti plasmid is derived from Agrobacterium tumefaciens, which is a plant pathogen. This plasmid is commonly used as a vector to transfer foreign genes into plant cells in genetic engineering applications.
A cosmid has the characteristics of a hybrid plasmid. They are used in the building of genomic libraries. They are ~200 base pairs long. More detailed information is available on Wikipedia.
it is a ds DNA use in recombinant DNA technology to insert our interested gene and multiply it.Ex;plasmid,cosmid
The insert capacity of a cosmid vector is about 35-45 kb.
Recombinants take up the plasmid but do not incorporate it into the DNA. Transformants take up the plasmid and integrate it into the DNA. Recombinants wouldn't express the new genes while Transformants will.
Plasmid DNA is an extra-chromosomal DNA molecule, it cannot link up with chromosomal DNA, and it contains the genetic informations that are necessary for its own replication. Episomes is any kind of extra-chromosomal DNA that can link up with chromosomal DNA. That is the main difference between them two. Episomes are usually larger than other extra-chromosomal DNA. An example of episome are the viruses, because they intergrate their genetic material into the host's chromosomal DNA.The only difference between the plasmid and the episome is the integration i-e plasmid can not integrate while episome can integrate into the genome.
Orginal Plasmids are extra chromosomal genetic material present in eukaryotes and some prokaryotes.Recombinant plasmids contain a gene of intrest ie,individual gene carrying a specific function can be inserted in to a specific site on original plasmid in cell culture via transformation.So the recombinant plasmid contain both gene of intrest and native genes.
Plasmid
The first step is restriction of the cosmid and the foreign DNA with the restriction enzyme, then ligating the fragments together. Thereafter, the cosmids are loaded into the phage capsid, which leads to the expression of the foreign gene through transduction.
R-plasmid
TOL plasmid
plasmid
The Ti plasmid is derived from Agrobacterium tumefaciens, which is a plant pathogen. This plasmid is commonly used as a vector to transfer foreign genes into plant cells in genetic engineering applications.