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What is a small double stranded DNA that's used as a vector?
it is a ds DNA use in recombinant DNA technology to insert our interested gene and multiply it.Ex;plasmid,cosmid
Why is cloning an example of cloning?
Gene Cloning is used to clone a gene of interest in a vector called plasmid. The chimeric DNA or rDNA formed by cloning is stable and can be used to propagate and sequence the DNA. producing vector containing inulin gene is an example.
What is orf in plasmid vector and role in cloning of gene of interest?
ORF stands for Open Reading Frame, which is a sequence of nucleotides that can be translated into a protein. In a plasmid vector, an ORF can be used to clone a gene of interest by inserting the gene sequence into the ORF region, allowing the production of the corresponding protein. The ORF acts as a template for protein synthesis, enabling the expression of the cloned gene in a host organism.
Significance of vector in DNA recombinant technology?
Vector are plasmid DNA, act as a molecular vehicles to carry genes or DNA of interest. In rDNA technology vectors used to clone the gene by ligation. This chimeric DNA or plasmid can be propagated in E.coli as the vector carries its own origin of replication. Expression plasmid vectors can be used to produce proteins from the gene of interest.
How can one effectively clone a gene into a plasmid?
To effectively clone a gene into a plasmid, the gene of interest and the plasmid are cut with the same restriction enzymes to create compatible ends. The gene is then inserted into the plasmid using DNA ligase to seal the ends. The plasmid is then introduced into a host cell, such as bacteria, where it can replicate and express the cloned gene.
In the process of human gene cloning using recombinant plasmids what is the bacterial plasmid?
The bacterial plasmid is a small circular DNA molecule that is used as a vector to carry the gene of interest in gene cloning experiments. It is introduced into bacteria, where it replicates independently from the bacterial chromosome. The gene of interest is inserted into the plasmid using restriction enzymes and ligase.
What is a Self replicating DNA used to transmit a gene from one organism to another?
Self-replicating DNA, such as a plasmid, is used in gene transfer techniques like bacterial transformation. The gene of interest is inserted into the plasmid, which can then replicate independently within a host cell, allowing for the transfer of the gene to another organism. This method is commonly used in genetic engineering to introduce new traits or gene functions into recipient organisms.
If you place gene in reverse orientation how can it be detected?
Most genes contain restrictions sites. Once you've inserted your gene into the vector you use restrictions sites in the gene and the vector to cut the vector into smaller pieces. If the pieces correspond to the pattern you expect for a reverse orientated gene then you know it is in the reverse orientation.
How do you predict a PCR product size from a gene cloned into an expression vector?
It all depends on where you primers are. Presumably you will have one primer that sits on the cloned gene and one that sits on the vector (that way you only get a product if the gene has cloned successfully). As long as you know where your primers land, it should be easy to work out how big the PCR product will be simply by adding the distance from the primer on the gene to the end of the gene and the distance from the primer on the vector to the end of the vector.
How can a virus conduct genetic engineering?
A virus can conduct genetic engineering by using it to insert a gene into a cell. The virus containing the gene is called a vector. By changing the cell, you can introduce to other cells.
What is the role of Cloning Vector in recombinant DNA technology?
A cloning vector is a DNA molecule used to carry a foreign DNA fragment into a host cell for replication. It serves as a vehicle for the insertion of DNA fragments and allows for the propagation of recombinant DNA. Cloning vectors typically contain sequences for replication, selection, and insertion of foreign DNA.
What is a positive selection vector?
A positive selection vector is a type of vector used in molecular biology that contains a gene conferring a specific trait or resistance to a selection agent, such as an antibiotic. This allows for the selection of only those cells that have successfully taken up the vector and integrated the gene.
