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Why maltose is used in phage titration?
Maltose is used in phage titration because some bacteriophages use maltose as a carbon source. By including maltose in the diluent, the phages are provided with the necessary nutrients to replicate and form visible plaques on a bacterial lawn, which can then be counted to calculate the phage titer.
Why the titration flask need to be shake during titration?
Shaking the titration flask during titration helps to ensure that the reaction mixture is well-mixed and that the titrant is evenly distributed throughout the solution. This promotes a more uniform reaction and more accurate measurement of the endpoint of the titration.
What are the disadvantages of potentiometric titration?
Some disadvantages of potentiometric titration include the need for specialized equipment such as a pH meter or ion-selective electrode, which can be costly. Additionally, it may require a skilled operator to perform the titration accurately and interpret the results correctly. Potentiometric titration can also be slower compared to other titration methods.
Why need to add reagent drop by drop during titration?
Adding reagent drop by drop during titration allows for precise control of the reaction and helps prevent over-titration. This ensures that the endpoint is accurately determined and the titration results are as precise and reliable as possible.
What is a titration and what equipment would you need?
A titration is a technique used to determine the concentration of a substance in a solution by reacting it with a solution of known concentration. Equipment needed for a titration typically includes a burette, pipette, flask, indicator, and a stir plate.
Why to use soft agar in phage titration?
Phage can move easily in soft agar,it can bind with another bacteria after lysis
Why maltose is used in phage titration?
Maltose is used in phage titration because some bacteriophages use maltose as a carbon source. By including maltose in the diluent, the phages are provided with the necessary nutrients to replicate and form visible plaques on a bacterial lawn, which can then be counted to calculate the phage titer.
A virus that reproduces in a bacterium is called?
phage
What is another term for a lysogenic phage?
temperate phage
When phage attaches to a bacteria the phage injects what?
DNA single
The substance a phage leaves outside its host cell?
The substance a phage leaves outside its host cell is typically referred to as the "phage progeny" or "phage particles." These particles can include newly synthesized phage DNA and proteins, as well as the phage capsid that encapsulates the genetic material. This material can go on to infect other host cells and continue the phage replication cycle.
What is temperate phage superinfection immunity?
c. Repression of the phage genome - A phage coded protein, called a repressor, is made which binds to a particular site on the phage DNA, called the operator, and shuts off transcription of most phage genes EXCEPT the repressor gene. The result is a stable repressed phage genome which is integrated into the host chromosome. Each temperate phage will only repress its own DNA and not that from other phage, so that repression is very specific (immunity to superinfection with the same phage).Reference: http://pathmicro.med.sc.edu/mayer/phage.htm
Why the titration flask need to be shake during titration?
Shaking the titration flask during titration helps to ensure that the reaction mixture is well-mixed and that the titrant is evenly distributed throughout the solution. This promotes a more uniform reaction and more accurate measurement of the endpoint of the titration.
Is Phage the Untouchable banned in any formats?
Yes, Phage the Untouchable is banned in the Commander format.
Why bacillus anthracis susceptible to gamma phage?
Bacillus anthracis is susceptible to gamma phage because the phage has evolved to target and infect specific receptors on the surface of the bacterium. This interaction enables the gamma phage to inject its genetic material into the bacterium, hijacking its machinery to produce more phage particles and ultimately leading to the destruction of the bacterial cell.
What are the disadvantages of potentiometric titration?
Some disadvantages of potentiometric titration include the need for specialized equipment such as a pH meter or ion-selective electrode, which can be costly. Additionally, it may require a skilled operator to perform the titration accurately and interpret the results correctly. Potentiometric titration can also be slower compared to other titration methods.
When a phage attaches to a bacterium what does the phage inject and what stays attached to the cell?
When a phage attaches to a bacterium, it injects its genetic material (DNA or RNA) into the cell. The phage capsid (outer protein coat) typically stays attached to the cell surface during this process.
