Phage can move easily in soft agar,it can bind with another bacteria after lysis
Maltose is used in phage titration because some bacteriophages use maltose as a carbon source. By including maltose in the diluent, the phages are provided with the necessary nutrients to replicate and form visible plaques on a bacterial lawn, which can then be counted to calculate the phage titer.
the use of phage to kill bacteria causing disease may infected the heart-valve.
Yes, agar agar can expire. To determine if it is still safe to use, check the expiration date on the packaging. Additionally, inspect the agar agar for any signs of discoloration, unusual odor, or mold growth. If any of these are present, it is best to discard the agar agar to avoid any potential health risks.
By an acid-base titration with 0.1 N hydroxide on phenolphtaleine (colorless pH 8 -> 10 purple-blue). At that endpoint-pH level it is a tri-acidic or tri-protolytic weak acid so its Normality:Molarity ratio is 3:1 ( 3.0 N=1.0 M).
Hershey and Chase used radioactive sulfur-35 to tag phage proteins and radioactive phosphorus-32 to tag phage DNA in their experiments on bacteriophages.
Yes, some marine bacteria and fungi have been identified to possess the capability to degrade agar agar. These microorganisms secrete enzymes such as agarases that breakdown agar into simpler sugars, which they can then use as a source of energy.
Agar-agar can be used in vegan cooking as a gelatin substitute to make desserts like puddings, jellies, and fruit gels. It can also be used to thicken soups, sauces, and even as a binder in vegan burgers or meatballs. Additionally, agar-agar can be used to create vegan versions of traditional Asian dishes like agar-agar noodles or agar-agar sushi rolls.
Restriction modification systems, CRISPR defense, physical barriers such as the excretion of mucus or formation of complex outer-membrane sugar structures to block phage adsorption, and modifications of phage receptors are just some.
The common ratio for mixing agar agar powder with water is 1 tablespoon of agar powder to 1 cup of water. This ratio may vary depending on the specific recipe or intended use.
In precipitation titration, the formation of a solid precipitate is used to determine the endpoint of the titration, while in complexometric titration, a complex formation reaction is used to determine the endpoint. Precipitation titration is often used for specific ion determinations, while complexometric titration is used for determining metal ions by forming stable complexes with titrant.
MacConkey agar is yellow (due to the lactose in the agar). When an organism consumes the lactose, the agar turns purple. Purple indicates a positive test. If the organism doesn't use the agar, the agar stays yellow.
To get an accurate titration value, ensure that all reagents are standardized and accurately measured, use an appropriate indicator, perform the titration slowly and carefully, and repeat the titration for consistency. Calibration and proper maintenance of equipment are also important for accuracy.