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Which is more stronger in dissolving lipids chloroform or hexane and why?
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What is the function of chloroform in DNA extraction?
It solubalize lipids and protiens to remove them from DNA
What is the definition of lipophilic?
Having an affinity for or capable of dissolving in lipids.
Role of chloroform in DNA isolation?
It solubilizes lipids and a lot of proteins to remove them from the DNA.
What is the function of chloroform isoamyl alcohol?
Did u mean function of chloroform isoamyl alcohol in DNA extraction? Chloroform isoamyl alcohol is a type of detergent. It binds to protein and lipids of cell membrane and dissolve them. By this it will disrupt the bonds that hold the cell membrane together and cause it to breakdown. It then form complexes with these lipids and proteins, causing them to precipitate out of solution. Note that lipids and proteins are non-aqueous compound and DNA/RNA are aqueous compound. The detergent binds to non-aqueous compound.
What is the function of buffered phenol chloroform in DNA extraction?
Phenol and chloroform are very hydrophobic and are used to remove non-polar bio- molecules (proteins, fragments of membranes, lipids etc) in the extraction.
Which solvent has the greatest lipid solubility Why do you think this?
The best lipid solvents are Hexane, Ethyl Alcohol and Methyl Alcohol. This is because lipids are nonpolar and hydrophobic. Hexane has the highest hydrophobicity of any solvent and is thus the best at dissolving hydrophobic molecules.
What are good solvents for lipids?
Non-polar organic solvents such as detergents (i.e. soap, which is just a salt of fatty acids, and other surfactants), and more powerful chemical solvents such as benzene, chloroform, diethyl ether, or hexane.
Role of chloroform in RNA isolation?
it solubilize the lipids and protein and remove them.
What is the function of chloroform in DNA extraction?
It solubalize lipids and protiens to remove them from DNA
What is the definition of lipophilic?
Having an affinity for or capable of dissolving in lipids.
Role of chloroform in DNA isolation?
It solubilizes lipids and a lot of proteins to remove them from the DNA.
Which of macromolecules cannot dissolve in water?
Lipids are not dissolving,. They have a hydrophobic part
What is the function of chloroform isoamyl alcohol?
Did u mean function of chloroform isoamyl alcohol in DNA extraction? Chloroform isoamyl alcohol is a type of detergent. It binds to protein and lipids of cell membrane and dissolve them. By this it will disrupt the bonds that hold the cell membrane together and cause it to breakdown. It then form complexes with these lipids and proteins, causing them to precipitate out of solution. Note that lipids and proteins are non-aqueous compound and DNA/RNA are aqueous compound. The detergent binds to non-aqueous compound.
What is the function of buffered phenol chloroform in DNA extraction?
Phenol and chloroform are very hydrophobic and are used to remove non-polar bio- molecules (proteins, fragments of membranes, lipids etc) in the extraction.
Why is hexane an appropriate solvent for extracting fat from hamburger meat?
Hexane is a non-polar solvent. Fats are also non-polar substances. Fats are thus highly and rapidly soluble in hexane as compared to any other solvent. Therefore, if a total fat content determination is required, hexane is the best while not suitable for polar and partially polar lipids. For determination of total lipids including all polar, partially polar and non-polar fats, the best described system is Chlroform:Methanol (3:1).
Are lipids water soluable?
The lipids are a large and diverse group of naturally occurring organic compounds that are related by their solubility in nonpolar organic solvents (e.g. ether, chloroform, acetone & benzene) and general insolubility in water.
What is the generalization of solubility of lipids?
Lipids are soluble in nonpolar or organic solvents.
