Its Newcastle-under-Lyme in Staffordshire with 75,000 to 120,000 people (depending on definition). It lost its station in the Beeching Report but the city of Stoke-on-Trent which its suburbs run into does have a station.
It is likely that Dudley is the largest town in England without a railway station. It has a population of over 300,000.
Wakefield
Wednesbury Town railway station ended in 1964.
Wednesbury Town railway station was created in 1850.
Harlow Town railway station was created in 1842.
Alford Town railway station was created in 1848.
Alford Town railway station ended in 1970.
Ash Town railway station ended in 1948.
Ash Town railway station was created in 1916.
Abbey Town railway station was created in 1856.
Birkenhead Town railway station ended in 1945.
Birkenhead Town railway station was created in 1878.
Town Hall railway station was created in 1932.
Bicester Town railway station was created in 1987.