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The brightness and contrast have some impact on eyestrain, but the main cause of eyestrain from Computer Monitors is the refresh rate.

You should turn the refresh rate up as high as your monitor supports, especially if you are in an area with fluorescent lights. The default refresh rate for a monitor is 60 Hz, the same as fluorescent lights. With your monitor flickering at 60 Hz, and the lights flickering at 60 Hz (and not at the same time) the back-and-forth flickering, even though it can't be noticed, can cause considerable eye strain.

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