The Fine Adjustment knob is used for focusing HPO.
The Coarse Adjustment Knob is used for focusing LPO.
The Fine Adjustment knob is used for focusing HPO.The Coarse Adjustment Knob is used for focusing LPO.
The fine adjustment knob should be used only with the high power objective (HPO) because it allows for precise focusing on small details at higher magnifications. When using HPO, the working distance is significantly reduced, and using the coarse adjustment knob could lead to crashing the objective lens into the slide, potentially damaging both the slide and the lens. The fine adjustment knob provides the delicate control needed to achieve clear images without risk of contact.
The fine adjustment knob should be used only with the high-power objective (HPO) because it allows for precise focusing at high magnifications. Using the fine adjustment knob with lower-power objectives can result in the objective lens hitting the slide, potentially damaging both the lens and the slide.
Why should the fine adjustment knob be used only with the HPO
Why should the fine adjustment knob be used only with the HPO
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Why should the fine adjustment knob be used only with the HPO
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The fine adjustment knob should only be used with the high-power objective (HPO) in a microscope to achieve precise focusing without risking damage to the slide or the objective lens. Using coarse adjustment at high magnification can lead to the lens crashing into the slide, potentially breaking the slide or the lens. The fine adjustment allows for delicate, minor adjustments needed for sharp focus at high magnification. This ensures optimal clarity and detail while maintaining safety for the specimen.
The coarse adjustment knob is designed for use with low-power objectives, allowing for significant changes in focus. When using high power objectives (HPO), lowering the stage too much can lead to the objective lens contacting the slide, risking damage to both the lens and the slide. Therefore, using the fine adjustment knob with HPO ensures precise focusing without the risk of collision. Always start with low power to safely locate the specimen before switching to higher magnifications.
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