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Which of the antibiotic resistance does your plasmid contain?
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What type of gene is used to distinguish bacteria that carry a plasmid containing foreign DNA from those that don' t?
The plasmid that contains foreign DNA is engineered to also carry an antibiotic resistance gene. This antibiotic resistance gene codes for a protein that is able to inactivate an antibiotic thus keeping the cell alive. In the absence of the antibiotic resistance gene, the cells would not survive when exposed to an antibiotic. After transfection (the process of inserting the plasmid carrying the foreign gene into cells), the cells are gown in media containing an antibiotic. Cells that contain the plasmid (and therefore contain the antibiotic resistance gene) are able to survive in this medium. Cells that do not contain the plasmid (and therefore lack the antibiotic resistance gene) do not survive in this medium. The process described above is called selection
What type of plasmid is the source of the problem with antibiotic resistance?
R plasmid.
What is one way to determine whether a bacterial culture has received a recombinant plasmid?
The plasmid have a "reporter gene" inside it, generally resistance to specific antibiotic. the plasmid is transformed into bacteria that don't have resistance to that specific antibiotic drug, and than the cultured on a petri-dish that contain the antibiotic drug. Only bacteria that had receive the plasmid will have resistance and grow, all the other will die.
What is an extra loop of DNA that contains antibiotic resistance what gene?
Plasmid
True or false cells containing a plasmid with an antibiotic resistance gene will die in the presence of the antibiotic?
false
What type of gene is used to distinguish bacteria that carry a plasmid containing foreign DNA from those that don' t?
The plasmid that contains foreign DNA is engineered to also carry an antibiotic resistance gene. This antibiotic resistance gene codes for a protein that is able to inactivate an antibiotic thus keeping the cell alive. In the absence of the antibiotic resistance gene, the cells would not survive when exposed to an antibiotic. After transfection (the process of inserting the plasmid carrying the foreign gene into cells), the cells are gown in media containing an antibiotic. Cells that contain the plasmid (and therefore contain the antibiotic resistance gene) are able to survive in this medium. Cells that do not contain the plasmid (and therefore lack the antibiotic resistance gene) do not survive in this medium. The process described above is called selection
What type of plasmid is the source of the problem with antibiotic resistance?
R plasmid.
What structures contains genes for enzymes and antibiotic resistance?
plasmid
What is one way to determine whether a bacterial culture has received a recombinant plasmid?
The plasmid have a "reporter gene" inside it, generally resistance to specific antibiotic. the plasmid is transformed into bacteria that don't have resistance to that specific antibiotic drug, and than the cultured on a petri-dish that contain the antibiotic drug. Only bacteria that had receive the plasmid will have resistance and grow, all the other will die.
Is it true cells containing a plasmid with an antibiotic resistance gene will die in the presence of the antibiotic?
false
What is an extra loop of DNA that contains antibiotic resistance what gene?
Plasmid
True or false cells containing a plasmid with an antibiotic resistance gene will die in the presence of the antibiotic?
false
How does plasmid allow for antibiotic resistance?
A plasmid (conjugative plasmid) that has a resistance gene on it, can transfer itself to another bacterial cell (called conjugation) or assist in the transfer of a non-conjugative plasmid that has a resistance gene to another cell (called mobilization). Whichever way it happens, once the plasmid is transfered to the new cell, this cell too may show signs of resistance to the particular antibiotic. This is one of the ways of the speard of resistance amongst bacteria
What one plate would you first inspect to conclude that the transformation occurred successfully Why?
If you transform bacteria with a plasmid containing a selection marker (such as an antibiotic resistance gene) and plate the transformed bacteria on a plate suited for selecting for plasmid-containing bacteria (such as a plate containing an antibiotic that only those bacteria with antibiotic resistance can survive), then simply inspecting whether colonies are present on the plate will suffice in determining whether the transformation succeeded. If no colonies are found, that means no bacteria got the antibiotic resistance gene on the plasmid and the transformation was unsuccessful. If some colonies are found, that means some bacteria contain the plamis containing the antibiotic resistance gene and those colonies can the transformation was successful.
Why are plasmids pieces of DNA?
i think each plasmid piece codes for a specific function. for example antibiotic resistance shown by antibiotic sensitive cell after a piece of plasmid that is antibiotic resistant gene recombine with cell DNA.
Why the cell do not contain plasmid if they not grow in antibiotics?
If you do not give antibiotics, the plasmid may lost after a few generation. it is because bacteria thinks that the extra DNA (plasmid) is not needed as the antibiotic is not present. That is the reason for keeping the culture always in antibiotic selection pressure.
Why do some plasmids contain a gene for resistance to antibiotic?
Plasmid contain a few genes including antibiotic resistance genes .Bacteria are highly active metabolically and many mutation are produced in them . These genes are produced by evolution.
