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What type of gene is used to distinguish bacteria that carry a plasmid containing foreign DNA from those that don' t?
The plasmid that contains foreign DNA is engineered to also carry an antibiotic resistance gene. This antibiotic resistance gene codes for a protein that is able to inactivate an antibiotic thus keeping the cell alive. In the absence of the antibiotic resistance gene, the cells would not survive when exposed to an antibiotic. After transfection (the process of inserting the plasmid carrying the foreign gene into cells), the cells are gown in media containing an antibiotic. Cells that contain the plasmid (and therefore contain the antibiotic resistance gene) are able to survive in this medium. Cells that do not contain the plasmid (and therefore lack the antibiotic resistance gene) do not survive in this medium. The process described above is called selection
True or false cells containing a plasmid with an antibiotic resistance gene will die in the presence of the antibiotic?
False. Cells containing a plasmid with an antibiotic resistance gene will survive in the presence of the antibiotic because they can produce the protein that confers resistance, allowing them to withstand the antibiotic's effects.
A gene for antibiotic resistance may be used as a making it possible to identify a transformed cell?
Yes, a gene for antibiotic resistance can be used as a selectable marker in transformation experiments. By incorporating the gene into a vector along with the gene of interest, researchers can grow the transformed cells on media containing the antibiotic, allowing only the cells that have successfully incorporated the gene of interest to survive. This method helps in identifying and isolating the transformed cells.
How does plasmid allow for antibiotic resistance?
A plasmid (conjugative plasmid) that has a resistance gene on it, can transfer itself to another bacterial cell (called conjugation) or assist in the transfer of a non-conjugative plasmid that has a resistance gene to another cell (called mobilization). Whichever way it happens, once the plasmid is transfered to the new cell, this cell too may show signs of resistance to the particular antibiotic. This is one of the ways of the speard of resistance amongst bacteria
Which type of adaptation develops quickly in bacteria that are antibiotic resistant?
Horizontal gene transfer is a type of adaptation that allows bacteria to acquire antibiotic resistance genes quickly from other bacteria. This process involves the transfer of genetic material between different bacteria, enabling the rapid spread of antibiotic resistance within a population.
What type of gene is used to distinguish bacteria that carry a plasmid containing foreign DNA from those that don' t?
The plasmid that contains foreign DNA is engineered to also carry an antibiotic resistance gene. This antibiotic resistance gene codes for a protein that is able to inactivate an antibiotic thus keeping the cell alive. In the absence of the antibiotic resistance gene, the cells would not survive when exposed to an antibiotic. After transfection (the process of inserting the plasmid carrying the foreign gene into cells), the cells are gown in media containing an antibiotic. Cells that contain the plasmid (and therefore contain the antibiotic resistance gene) are able to survive in this medium. Cells that do not contain the plasmid (and therefore lack the antibiotic resistance gene) do not survive in this medium. The process described above is called selection
True or false cells containing a plasmid with an antibiotic resistance gene will die in the presence of the antibiotic?
False. Cells containing a plasmid with an antibiotic resistance gene will survive in the presence of the antibiotic because they can produce the protein that confers resistance, allowing them to withstand the antibiotic's effects.
Antibiotic in gene cloning?
If antibiotic resistance is added to the gene being cloned, antibiotics can be used to isolate the transformed bacteria (ones with the gene being cloned) by killing off all non-transformed bacteria, that don't have the antibiotic resistance. There is a chance that the non-transformed bacteria can mutate to develop antibiotic resistance.
What can gene for antibiotic resistance be used for?
genetic marker
A gene for antibiotic resistance may be used as?
Genetic Marker
Is it true cells containing a plasmid with an antibiotic resistance gene will die in the presence of the antibiotic?
false
Why do plasmids contain a gene for antibiotic resistance?
Plasmids contain antibiotic resistance genes because these genes provide a survival advantage to the bacteria in the presence of antibiotics. Bacteria can pick up plasmids with antibiotic resistance genes through horizontal gene transfer, allowing them to survive in environments with antibiotic exposure. This is a common mechanism for bacteria to acquire resistance traits and poses a challenge for antibiotic treatment.
How are cells screened to determine if the recombinant DNA was taken up?
Usually recombinant DNA is packaged in a plasmid that contains a marker gene. This marker can be an antibiotic resistance gene (NPTII for Kanamycin) or a gene that enables the plant to synthesise an amino acid. For antibiotic resistance the cells are grown on a medium that contains the antibiotic. The ones that grow have the marker gene. Sometimes the cells are transformed with a mixture of plasmids, some with the target gene and some without. The LAC-operon is used to select the cells that have the gene inserted. The gene-insertion inactivates the LAC-Z gene. Cells grown on X-gal plates will be blue, unless there's a transgene present. So white colonies have the transgene.
A gene for antibiotic resistance may be used as a making it possible to identify a transformed cell?
Yes, a gene for antibiotic resistance can be used as a selectable marker in transformation experiments. By incorporating the gene into a vector along with the gene of interest, researchers can grow the transformed cells on media containing the antibiotic, allowing only the cells that have successfully incorporated the gene of interest to survive. This method helps in identifying and isolating the transformed cells.
How do ampicillin resistant E. coli combat the lethal effects of the ampicillin antibiotic?
The pGlo plasmid contains an ampicillin resistance gene. This gene encodes an enzyme, β lactimase, which enzymatically degrades ampicillin. Therefore, bacteria that take up the plasmid (transformants) become resistant to ampicillin.
How does plasmid allow for antibiotic resistance?
A plasmid (conjugative plasmid) that has a resistance gene on it, can transfer itself to another bacterial cell (called conjugation) or assist in the transfer of a non-conjugative plasmid that has a resistance gene to another cell (called mobilization). Whichever way it happens, once the plasmid is transfered to the new cell, this cell too may show signs of resistance to the particular antibiotic. This is one of the ways of the speard of resistance amongst bacteria
The use of an antibiotic resistance gene on a plasmid used in genetic engineering makes?
direct selection possible
