The lagging strand
When the template strand of DNA is read from 3' to 5', DNA synthesis occurs in the 5' to 3' direction.
Transcription
Following the initiation of DNA replication, the first step is the synthesis of a short RNA primer.
The product of a DNA synthesis reaction is a newly synthesized DNA strand complementary to the template DNA strand. This process involves the incorporation of nucleotides by DNA polymerase to form a double-stranded DNA molecule.
If DNA ligase were defective, it would impede the ability to join Okazaki fragments during DNA replication. This could result in gaps in the newly synthesized DNA strand, leading to mutations and potential disruptions in vital cellular processes.
A molecule of RNA complementary to the coding strand DNA in a gene is called messenger RNA (mRNA). mRNA is transcribed from the DNA template strand and carries the genetic information from the DNA to the ribosome for protein synthesis. It is made up of nucleotides that are complementary to those on the coding strand of DNA.
dNTPs are the building blocks of DNA that allow for the synthesis of new DNA strands during replication. They contain all four bases (A, T, C, G) and have a hydroxyl group at the 3' carbon, which allows for further elongation of the DNA strand. On the other hand, ddNTPs are chain-terminating nucleotides that lack the 3' hydroxyl group. When incorporated into a growing DNA strand by DNA polymerase, they prevent further elongation, leading to the termination of DNA synthesis. In summary, dNTPs facilitate DNA synthesis by adding nucleotides to the growing strand, while ddNTPs inhibit DNA synthesis by terminating the strand.
The leading strand would utilize the 3' to 5' template DNA strand as a guide for continuous synthesis of complementary DNA in the 5' to 3' direction by DNA polymerase during DNA replication.
The process is called DNA replication. It begins with the unwinding of the DNA double helix by an enzyme called helicase. Each separated strand then serves as a template for the synthesis of a new complementary strand by DNA polymerase.
You would need a DNA polymerase protein to complete the synthesis of a new strand of DNA. DNA polymerase is an enzyme that assembles new DNA strands by adding nucleotides one by one in the 5' to 3' direction.
The leading strand in DNA replication serves as a template for the continuous synthesis of a new complementary strand of DNA. It is replicated in a continuous manner by DNA polymerase, allowing for efficient and accurate replication of the entire DNA molecule.
during the S phase of the cell cycle, where the DNA double helix unwinds and each strand serves as a template for the synthesis of a new complementary strand. Enzymes called DNA polymerases catalyze the formation of phosphodiester bonds between nucleotides to build the new DNA strand. The end result is two identical DNA molecules, each consisting of one original strand and one newly synthesized strand.