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What Reagents are used in osazone test?
Reagents of Osazone test are 1. Phenylhydrazine mixture consisting of phenyl-hydrazine and anhydrous sodium citrate 2. Orignal solution containing a carbohydrate
What is the clinical significance of osazone test?
For identification purposes the carbonyl and adjacent alcohol functions will form phenylhydrazine derivatives known as osazones, which give characteristic melting points and exhibit definite crystalline structure. It should be noted that glucose , fructose and mannose yield the same osazone since the difference in structure and configuration about carbon atoms 1 and 2 are abolished.
What is the importance of phenylhydrazine test?
The phenylhydrazine test is also known as the Osazone test. The phenylhydrazine test is used for reducing the amounts of carbohydrates, momosacchstides, and disaccharides.
Why glucose and fructose form same osazone?
Glucose, Fructose and Mannose give the same osazone because these sugars have the same configuration of carbons 3, 4, 5 and 6. osazone formation only affects carbons 1 and 2.
Osazone test for carbohydrates?
osazone test is a carbohydrate specific reaction in which carbs react with phenylhydrazine to form osazones i.e. crystals of specific shape. thus the test confirms the presence of a particular sugar.Eg:for sucrose one gets a mixture of glucosazone and fructosazone on the basis of components of sucrose.
Why glucose and fructose can be identified through osazone crystal formation?
They are both reducing sugars. They have aldose and ketose group at the side of the structure, which helps the sugar to condense with phenylhydrazine and produce solid derivatives called osazone. The solid is seen as crystals through the microscope.
After hydrolysis did sucrose give a positive test with fehlings test why orwhy not?
After hydrolysis sucrose did give a positive test. It reduces sugar that has hemiketal and hemiaetal in it.
What are two aldotetroses that yield the same osazone on reaction with phenyl hydrazine?
D-erythrose and D-threose both yield the same osazone. Likewise, L-erythrose and L-threose yield the same osazone.
Why osazone obtained from D-glucose and D-fructose have the same melting points?
glucose and fructose form same osazone crystals because carbon no 1 to carbon no 6 are identical both in glucose and in fructose
Why you used sodium acetate in osazone test?
Anhydrous sodium acetate is added to buffer the phenylhydrazine HCl so that it may function properly. Regards mawaisnawaz@hotmail.com
Why do people with diabetes have to test their blood sugar level before injecting themselves insulin?
Once you test your blood sugar, you can figure how much insulin to give yourself. Because your blood sugar varies every time you check, that is why.
Why does glycoproteins reacts positively with Molisch reagent?
Glycoproteins give positive results for Molisch's test. This answer is true because Molisch test was a test for sugar.
