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two identical DNA fragments will have identical restriction fragments.
Also, genetically identical twins will have identical restriction fragments
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∙ 13y ago
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Does a restriction enzyme generate the same size fragments in genomic DNA of different species?
No. A restriction enzyme cuts DNA when it finds a specific sequence. Different animals will have these sequences occur at different intervals so the length of the fragments won't be the same.
How are restriction maps used?
They are used to show the lengths of DNA fragments between restriction sites in a strand of DNA.
What cuts DNA into fragments?
cutting of DNA into fragments simply means application of suitable restriction enzyme to it.now a days two types of restriction enzymes are available,1)exonucleases,which cut at end portion of DNA and 2)endonucleases ,which cut at specific inner site.
What are used to cleave DNA into fragments?
Restriction enzymes. Babe
If you took a linear piece of DNA and cut it with the restriction enzyme EcoRI and it had three restriction sites for EcoRI, how many fragments would you produce What if you had a circular piece of DNA?
If the plasmid has 3 recognition sequences for a given restriction endonuclease, then 4 linear DNA fragments are obtained because, if the DNA is linear then the number of fragments obtained is (N+1) whereas if the DNA is circular then the number of fragments obtained will be N for N recognition sequences for the given restriction endonuclease in a plasmid.
How do restriction sites and a restriction map relate?
Restriction maps show the lengths of DNA fragments between restriction sites in a strand of DNA.
What is the simple rule relating the number of fragments to the number of restriction sites presents on the linear DNA molecule?
Let N = number of fragments: For circular DNA, the number of restriction sites= N For linear DNA, the number of restriction sites= N-1
Which protein creates DNA fragments with sticky ends?
Restriction Enzyme
What is a collection of cells containing DNA fragments produced by restriction enzymes and incorporated into plasmids?
A DNA LibraryA collection of cells containing DNA fragments produced by restriction enzymes and incorporated into plasmids is called a DNA library. RNA can manufacture DNA via the action of reverse transcriptase.
Why was the discovery of restriction enzymes important to recombinant DNA technology?
Recombinant DNA technology requires fragments of DNA from the source genome. Using crude methods such as mechanical shearing, we get random fragments of DNA, and their sequence is unknown. Restriction enzymes are specific in site recognition and cutting and their discovery lead to proper fragments of DNA which have some known sequences.
What are enzymes cutting DNA at specific sites to form restriction fragments called?
restriction enzymes or endonuclease enzymes
What is created by treating DNA with restriction enzymes to create fragments allowing them to travel in an agarose gel bed with electricity and capturing the results in a type of picture?
When DNA is treated with restriction enzymes, and the fragments are loaded onto a gel which is subjected to electrophoresis, we get a banding pattern of the DNA fragments with the farthest band (from the gel) of those fragments smallest in size.
