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What cell walls are you using? In mammalian cell culture the reason you would use Ca/Mg free PBS is to help remove them from the surface they are attached to. The adherens proteins that adherent cells use to stick to whatever they are stuck to requires Ca2+ and Mg2+. Therefore if you simply want to wash the cells you have to use a buffer WITH Ca2+ and Mg2+.
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∙ 15y ago
Calcium and magnesium-free PBS is often used in cell culture studies to prevent interference with cell signaling pathways or enzyme activities that can be affected by these divalent cations. Removing calcium and magnesium from the PBS helps maintain stable conditions for the cells to thrive and minimizes potential confounding factors in experiments.
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Can calcium displace from magnesium?
As calcium is more reactive than magnesium calcium will displace the magnesium and will become calcium nitrate and is left over with magnesium on it's own
What is the gas that reacts with calcium and magnesium when they burn?
The gas that reacts with calcium and magnesium when they burn is oxygen. This reaction forms calcium oxide and magnesium oxide as the products.
Would magnesium react with calcium nitrate?
no, it wouldn't calcium is above magnesium in the reactivity series which means that magnesium can't oxidize calcium. calcium can oxidize magnesium so calcium would react with magnesium nitrate with formation of metallic magnesium and calciumnitrate.
Would calcium nitrate and magnesium react?
Calcium nitrate and magnesium will not react since calcium is more reactive than magnesium and so, magnesium does not displace calcium (See Reactivity Series). However, if Calcium nitrate is in aqueous solution (dissolved in water), some bubbles may be seen when Magnesium is added to it, since the Magnesium would be reacting with the water and producing hydrogen bubbles.
How does the metal magnesium differ from the metal calcium in their reaction with water?
Magnesium reacts with water to produce magnesium hydroxide and hydrogen gas, while calcium reacts with water to produce calcium hydroxide and hydrogen gas. Both metals displace hydrogen from water due to their higher reactivity, but magnesium reacts more vigorously than calcium.
Why do you wash cells with PBS before adding trypsin?
Because PBS removes Magnesium and Calcium ions which inhibit trypsin.
What is the differences between DPBS and PBS?
DPBS (Dulbecco's Phosphate Buffered Saline) contains calcium and magnesium ions which are essential for cell growth and function, making it suitable for cell culture applications. PBS (Phosphate Buffered Saline) lacks these ions and is primarily used in biological research for rinsing and diluting biological samples. DPBS is typically preferred for maintaining cell viability in cell culture experiments, while PBS is commonly used for washing and buffer solutions in experimental protocols.
Is calcium with magnesium better for you?
A mixed calcium and magnesium supplement (such as dolomite) is better than taking just calcium or just magnesium.
Can calcium displace from magnesium?
As calcium is more reactive than magnesium calcium will displace the magnesium and will become calcium nitrate and is left over with magnesium on it's own
What is least reactive calcium magnesium radium strontium and barium?
calcium
What is the gas that reacts with calcium and magnesium when they burn?
The gas that reacts with calcium and magnesium when they burn is oxygen. This reaction forms calcium oxide and magnesium oxide as the products.
What 2 elements make water hard?
magnesium and calcium.
Would magnesium react with calcium nitrate?
no, it wouldn't calcium is above magnesium in the reactivity series which means that magnesium can't oxidize calcium. calcium can oxidize magnesium so calcium would react with magnesium nitrate with formation of metallic magnesium and calciumnitrate.
What is the difference between calcium hydroxide and Magnesium hydroxide?
Calcium hydroxide and magnesium hydroxide are both metal hydroxides used in various applications. However, calcium hydroxide is more commonly known as slaked lime and is used in construction, agriculture, and food industry while magnesium hydroxide is primarily used as an antacid to treat indigestion and heartburn. Additionally, magnesium hydroxide is a white solid whereas calcium hydroxide is a white powder.
What is the measurement of the amount of calcium and magnesium which water contains?
The amount of calcium and magnesium in water determines its hardness.
Where does calsium and magnesium come from?
Calcium and magnesium are naturally occurring elements that are commonly found in mineral deposits, rocks, soil, and water sources. They are essential nutrients for plant and animal health, and are typically obtained through dietary intake from sources such as dairy products, leafy green vegetables, nuts, seeds, and certain types of fish.
Would calcium nitrate and magnesium react?
Calcium nitrate and magnesium will not react since calcium is more reactive than magnesium and so, magnesium does not displace calcium (See Reactivity Series). However, if Calcium nitrate is in aqueous solution (dissolved in water), some bubbles may be seen when Magnesium is added to it, since the Magnesium would be reacting with the water and producing hydrogen bubbles.
