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DNA duplex strands are bonded by hydrogen bonds. On heating the hydrogen bonds break. At specific temperature called Tm half of the double helix is broken down (separated from each other) while the other half remains as such. This temperature can be determined , also the GC bond is stronger than AT so in such cases the temperature is more if the helix has more GC bonds.

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Q: Why DNA duplex melts at a specific temperature on heating?
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