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What is the principle behind Quantification of DNA by reading its optical density at 260nm and 280nm?
It is to check the purity of the DNA sample where it may have the interaction of RNA and sheared DNA in it.
What does protease do to DNA?
It cooprates with the lysis buffer to make our DNA sample visible to the human eye! lol jk.
Why extraction buffer is added for isolation of DNA?
Extraction Buffer is used to maintain pH of the solution.which prevents denaturation of DNA.
What is the role of Ficol in DNA quantification?
Ficoll, basically polysucrose is used to prepare density gradients during centrifugation to separate DNA fragments.
What is used to create a large sample of DNA for testing for a small sample of DNA?
PCR
Why dilute the DNA in TE?
TE is used as storage buffer
Role of Cl in TE buffer solution?
TE buffer is a often used as a buffer solution in molecular biology, mainly in procedures involving DNA or RNA. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
What is TNE buffer?
tris, EDTA (TE solution) and NaCl, TNE buffer is a buffer solution used in molecular biology, especially for DNA and RNA
What is the function of tritonx100 in DNA extraction?
Triton X-100 is used as a lysis buffer for DNA separation.
What does buffer aw1 and aw2 do?
Buffer AW1 contains Guanidinium Chloride (guanidine hydrochloride). This is used to denature proteins in your sample. They will then flow through the column and will be discarded with the wash. Buffer AW2 is essentially 70% EtOH. 30 mls of 100% EtOH is added to the 13 mls of "concentrate"included in the bottle. 70% EtOH is used to remove salts from your column and aid in purifying your DNA.
Why is buffer HB used in DNA extraction?
It serves to break the tissue apart so the DNA can be subsequently extracted.
What is the function of CL buffer in DNA extraction?
In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
