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Check the ingredient i.e. salt concentration and also check whether the blood cell get lysed as some of the microbes need lysed blood cell and some need intact so depends upon needs of bacteria check your case and also see if you have taken a culture which should not be too old.

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Q: Why are my blood agar plates showing no growth?
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Related questions

What type of media is used for growing a culture from a throat specimen?

blood and chocolate agar plates as well as in universities laboratory Nutrient agar plates are also provided


What if growth appears on one of the unopened plates?

agar was contaminated


How do you store sheep's blood agar plates?

in the fridge


Agar media required for growth of Neisseria meningitidis?

sheep blood agarchocolate agar


How will you check the sterility of the media?

Agar is a medium, so you are checking the sterility of the agar. After preparation one usually places an agar plate at room temperature and another agar plate at 35 to 37 degrees C. After 24-48 hrs of incubation a visual check is made to see if there is any visible growth on the uninoculated plates. If you are adding blood, etc. to the agar, those components can be checked by subbing them to a blood agar plate to see if there is any growth--which would indicate non-sterile components.


How are Petri dishes sterilized?

To determine that an agar plate is sterlize you can keep it overnight in incubator without streaking and if there is any growth peresent in it then it is contaminated and if not then it is obveiously sterlize


Does pseudomonas aeruginosa grow on blood agar?

Yes, Pseudomonas aeruginosa can grow on blood agar, but it may not show the typical hemolysis patterns like other bacteria. It usually appears as flat, greenish colonies on blood agar plates.


What is alger plates?

Do you mean "agar plates"? Agar agar is a seaweed that, when boiled, makes a product similar to gelatin. This stuff is edible, so vegans who liked jello can have it. It's used by boiling it in water, adding things to it, pouring it into petri dishes and letting it harden to use in making bacteria cultures. Some of the things that are added to it are beef broth--"nutrient agar"--and blood--"blood agar."


Why is a TSA used for the streak plate?

Tryptic soya agar plates are used for streaking as it is a very general agar (ie not selective) and it promotes the growth of a broad spectrum of micoorganisms


What is responsible for solidifying nutrient agar plates?

Agar


Is blood heart infusion agar a defined or a complex medium?

Blood agar is enriched medium which are complex media to which an extra nutrient source (such as blood in this case) is added to encourage the growth of fastidious heterotrophs.


Is moraxella catarrhalis haemolytic?

Morazella catarrhalis is non-hemolytic, but will grow on chocolate blood agar plates.