we use sterile water because to avoid false result by microorganism having in the water and same reason for distilled also to avoid impurities like crystals of some metal ions and also to avoid other microorganisms
Brightfield microscopy is commonly used to visualize stained specimens. This type of light microscopy relies on illumination from below the specimen, making it possible to observe the contrast between specimen and background. Staining helps enhance this contrast by highlighting specific structures or components within the specimen.
Stains.
Commonly a petri dish, although there are many other mediums...even chicken broth!
The general term is 'stain' of which there are several chemicals that can be used, depending on the specimen.
Phase-contrast microscopy is the observation of internal structures of living microbes where as bright field microscopy is the observation of killed stained specimens and naturally colored live ones.
The clinical specimens that are gram stained are microorganism, bodily fluids and sputum specimen. Gram staining makes it easier for the identification of organisms.
That is not the case; you are looking at how some dissection specimens have had their veins and arteries stained. It is the dyes used in the preparation of the specimens.
molecular biology
Specimens are stained in sterile water to prevent contamination of the sample, which could affect the staining process and lead to inaccurate results. Staining in sterile water helps maintain the integrity of the sample and ensures that the staining procedure is carried out under controlled conditions.
Brightfield microscopy is commonly used to visualize stained specimens. This type of light microscopy relies on illumination from below the specimen, making it possible to observe the contrast between specimen and background. Staining helps enhance this contrast by highlighting specific structures or components within the specimen.
Live specimens cannot be used in a transmission electron microscope (TEM) as the high vacuum environment and electron beam would quickly kill any living cells. Instead, biological specimens must be fixed, dehydrated, and stained prior to imaging in a TEM.
Specimens viewed with a compound microscope need to be thin to allow light to pass through and be focused by the lenses. Staining with dyes increases contrast and visibility of certain structures within the specimen by highlighting specific parts of the sample. This helps to distinguish different components and makes them easier to observe under the microscope.
Stains.
Biopsy specimens are often sliced into thin slices, stained, mounted on a glass slide, and examined using a light microscope. Newer sample preparation techniques involve the rapid freezing of the sample and slicing of the still-frozen material.
Specimens need to be thin to allow light to pass through and enable clear visualization under the microscope. Staining with dyes helps to highlight specific structures or components within the specimen, making them easier to observe and distinguish.
No. Not unless it is damaged or stained. And sometimes not even then.
Dead specimens must be used with transmission electron microscopes because living cells are easily damaged by the high-energy electron beam used to create the image. Dead specimens can be fixed and stained to preserve their structure for imaging without being affected by the electron beam. Additionally, dead specimens allow for more control over sample preparation and consistency in imaging.