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The nucleotides also called dNTPs are free nucleotides that are used in the replication of new strands. The complete master mix contains Taq DNA polymerase, deoxynucleotides, oligonucleotide primers, magnesium ions, and buffer.

Function:

Taq DNA-polymerase: Enzyme is heat-resistant as it can withstand the highest temperature during the PCR process.

deoxynucleotides (dNTPs): Nucleotides that are used in the replication of new strands.

Oligonucleotide primers: Pieces of DNA complementary to the template that tell DNA

polymerase exactly where to start making copies.

Magnesium ions: A cofactor (catalyst) required by DNA polymerase to create the

DNA chain.

Salt buffer: Provides the optimum ionic environment and pH for the PCR reaction.

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Q: Why are there nucleotides A T G and C in the master mix and what are the other components and what are there functions?
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