Caffeineis dule absorbance,it gives multiwavelength responds like 205,245,273.
Moles = Mass / Mr. In this case Moles = 35.5 / Mr. of Caffeine The Mr. of caffeine (C8H10N4O2) is 194. Moles = 0.183 (rounded)
Caffeine having different wavelents it having 2 maximas and 1 minima. 1st maxima is 205nm 2nd maxima is 273nm minima is 245nm and it is primary reference standard and also suggested in pharmacopiea. -Rajesh,Orchid
Calibration standards are used to record the accuracy of certain calibrations that are performed. The accuracy allows the user to find out how good the calibration was.
vanilline - 81°C to 83°C (range-1.5°) Acetanilide - 164°C to 165.7°C (range-1.0°) Caffeine - 235.6°C to 237.5°C (range-1.0°) All three standards starting & Ending points is within 1.5° only. In our operational way also it covers minimum, middle & Maximum calibration covered.
sources used for instrument checking and calibration irradiation of other materials, and radiography called
Internal calibration is a process used inside a system to make fine adjustments. It is commonly used in equipment and vehicles to maintain accuracy.
With each objective, the calibration must be recalibrated because the calibration only applies to its calabrated objective.
Calibration gases are used to ensure that gas detectors are operating properly when working in confined spaces. They are commonly used in underground utilities or natural gas refineries.
Calibration weights are used to weigh things. They come in masses such as a 100g or 500 weight. They are used to exactly and accurately measure objects. They are placed on a scale and are then used to weigh the object.
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
propyl paraben being a stable compound with reproducible chromatographic parameters used as a internal standard for the calibration of the HPLC systems