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If you mean magnification, unless you have a confocal microscope, the objective lens with the lowest mag will be the shortest -- largest clearance between the bottom of the lens and the stage. You can easily load and unload (slide in or out) the sample off the stage. If the reason is the above, I have a better way. I always load the sample with the lens rotated away -- the best clearance will be when no lenses are in position. If so, I would have lowered the stage quite a bit so that when I swing the lens in position, I am not scratching the sample.
However, a better reason for starting with low mag would be that you can focus much easier with the lowest-mag lens and then refocus to go to a higher mag -- the sample is approximately in focus all these times. When the sample is way out of focus, as when the highest-mag lens is on when you first insert the sample, it is hard to know which direction to turn the focus knob without scratching the sample.
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