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Because it's a relative value.
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∙ 15y ago
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Why are the absorbance values obtained from the conversion chart rather than the absorbance scale on the spectronic 20?
because that chart gives a more accurate value than the absorbance scale on the specthometor
What is e-value of a optical brightening agent?
e-value of an optical brightening agent is the absorbance value obtained (in the UV region), when the absorbance of 1% solution is measured using a cuvette of 1 cm path length. T.J.R.PILLAI
What is the absorbance value for tartrazine?
427nm plus or minus 3 nm.
What is the absorbance value for thymol?
thymol blue 436, 545 and 595 nm
How do you find the concentration of potato cells immersed in salt solutions?
You would have to use a spectrophotometer to measure the absorbance of your unknown solution. But first, you need to make several solutions with known concentrations. Measure the absorbance of the known concentrations and plot them on an X and Y axis where X equals concentration and Y equals absorbance. Do a best-fit line for your data. Measure the absorbance of your unknown solution. Find this value on your Y-axis and find out where this value intersects with your line of best fit. The X value at the intersection is your concentration of potato cells. By the way, make sure you use the same wavelength throughout the experiment.
In a plot of absorbance vs concentration what does the slope represent?
The slope of absorbance vs concentration reptresents the value of εb, where ε is the absorbtivity with units of (L/mol cm) and b is path length measured in cm.
What is AUFS in hplc?
absorbance units full scale
What is the theoretical absorbance at 340 nm of a 0.01 M solution of NADH?
A = Elc A = (6220 M^-1 cm^-1)x(1 cm)x(0.01 M) A = 62.2 (units cancel, so no units in absorbance)
Why are the absorbance values obtained from the conversion chart rather than the absorbance scale on the spectronic 20?
because that chart gives a more accurate value than the absorbance scale on the specthometor
What is e-value of a optical brightening agent?
e-value of an optical brightening agent is the absorbance value obtained (in the UV region), when the absorbance of 1% solution is measured using a cuvette of 1 cm path length. T.J.R.PILLAI
What is the absorbance value for tartrazine?
427nm plus or minus 3 nm.
What is the absorbance value for thymol?
thymol blue 436, 545 and 595 nm
Why the value of absorbance is always between the 0-2?
It's over 9000.
True or false As the concentration of a colored solution increases the absorbance value decreases?
Absorbance rises with concentration because there is more material for a given frequency of light to be absorbed in. Your statement is false.
Why should an absorbance level not exceed a value of 1?
Since the absorbance level is actually just a fraction of radiation absorbed at a given wavelength, it should never rise above %100.
What Beers law?
Beer's law says that absorbance of a molecule or solution is:A = a*b*cwhere A is the absorbance, "a" is the absorptivity (in units of per molar per cm, M-1 cm-1), "b" is the path length (in units of centimeters, cm), and "c" is the concentration (in units of molar, M). The absorptivity, is also commonly known as epsilon.That means that the absorbance is linearly proportional to the thickness of the sample, the concentration of the absorbing medium, and the absorptivity, which is a measure of a given molecule's ability of absorb light.See the Web Links for more information.
Is wavelength or absorbance the dependent variable?
"absorbance"Since in the experiment, you probably choose the wavelength, then measure the absorbance (absorption?, the absorbance is the dependent variable.
