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Rothera's test is used to detect the presence of ketone bodies in a solution (eg urine). it utilizes sodium nitroprusside, liquid ammonia and ammonium sulphate. ammonium sulphate is used to concentrate the ketone bodies to the center of the solution. the other 2 constituents react with ketone bodies to form pink coloured polymerized compound. Rothera's test is used to detect the presence of ketone bodies in a solution (eg urine). it utilizes sodium nitroprusside, liquid ammonia and ammonium sulphate. ammonium sulphate is used to concentrate the ketone bodies to the center of the solution. the other 2 constituents react with ketone bodies to form pink coloured polymerized compound. Ammonium ion is used to precipitate the proteins that may be present.. Because proteins can give a positive result to Rothera's test as well..
The Rothera nitroprusside test looks for ketone bodies in urine. It is done by taking 5 mL of urine and adding ten drops of concentrated ammonia water and solid ammonium sulfate. The sample is then analyzed after 15 minutes. If the sample turns purple, then that means there is acetoacetic acid in the urine.
ammonium hydroxidde
Rat urine will actually saturate the drywall. Drywall is very porous. The best bet is to cut out and replace that section.
no
Uric acid is a by product of protein digestion and can be found in the urine.
Specific gravity is determined by the solutes in the urine - the less solutes, the lower the specific gravity. In normal urine, the solutes are ions such as ammonium.
You only need enough to saturate the test pad. Put it in the stream of urine as directed, then when you are sure the urine hit the pad, you should have enough to perform the test.
The exact composition varies with diet and liquid consumption, but the main components are the following: water, urea, uric acid, creatinine, various ions (sodium, potassium, chloride, ammonium, calcium, magnesium, phosphate, sulphate, etc), hormones, fatty acids, amino acids.
See the related link. The basic process to recover magnesium ammonium phosphate hexahydrate from urine is; allow urine to age till pH is around 9-9.5, then a filter bag with magnesium oxide is circulated with the urine. The final product is in the filter bag.
it could saturate it, so the reading is unclear. I would do the test again to be sure.
Wohler rejected this theory by obtaining urea,an organic compound in the urine of mammals,from ammonium cyanate