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What is the need for centrifuge in DNA isolation?
The frist part of DNA isolation involves separating the nucleic acids from other cellular components. A centrifuge is required to partition proteins from the nucleic acids
What machine spins and is used to extract DNA from other molecules?
Its a centrifuge.
What does PBS do in DNA extraction?
Phosphate Buffered Saline (PBS): a salty solution of constant pH to keep tissues, cells, and proteins intact during maceration
What is used to create a large sample of DNA for testing for a small sample of DNA?
PCR
Role of saline tris edta in DNA extraction?
Its purpose is to isolate DNA from a protein mixture.
What is the function of the alcohol in DNA extraction?
DNA is not soluble in isopropyl alcohol. It will precipitate out when you add this solvent. Once out of solution you can centrifuge it down and collect the pellet of DNA.
What is the need for centrifuge in DNA isolation?
The frist part of DNA isolation involves separating the nucleic acids from other cellular components. A centrifuge is required to partition proteins from the nucleic acids
Double strand DNA separation from single strand DNA in centrifuge?
The two strands can not be separated mechanically.
What machine spins and is used to extract DNA from other molecules?
Its a centrifuge.
What does PBS do in DNA extraction?
Phosphate Buffered Saline (PBS): a salty solution of constant pH to keep tissues, cells, and proteins intact during maceration
What is used to create a large sample of DNA for testing for a small sample of DNA?
PCR
Role of saline tris edta in DNA extraction?
Its purpose is to isolate DNA from a protein mixture.
What are the uses of DNA cloning in Forensic Science?
To produce enough copies of a DNA sample, that identification can be made of the source of the DNA sample.
What is supernatant solutions?
Supernatant liquid is the upper layer of fluid found after a mixture has been centrifuged. Because of its lower density, the fluid and the components in it have a lesser tendency to migrate to the bottom of a centrifuge tube.
Which of the DNA typing techniques do you think you would choose if you had to analyze a DNA sample?
Which of the DNA typing techniques do you think you would choose if you had to analyze a DNA sample? Why?
Role of sodium chloride in DNA extraction?
Sodium chloride was needed to ensure the proteins in the cell aren't separated from the rest of the solution with the DNA.
When DNA profiling is carried out the second DNA sample the one not collected at the crime scene is called the?
mitochondrial sample
