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What is the purpose of double spotting the chromatography paper?
The chromatography paper is spotted twice so as to increase the amount of sample used for the separation on the paper. This is to allow distinct banding of the sample into its constituents.
Why must the boiling tube be stoppered by a cork in paper chromatography?
So that the harmful liquid does not come out and seriously injure you.
Uses for paper chromatography?
They are used in many scientific studies to identify unknown organic and inorganic compounds. They are also used in crime scene investigation, DNA and RNA sequencing, among others. Essentially, any solution can be separated through some form of chromatography.
What is the purpose of the solvent in chromatography?
The basic purpose of the solvent is to move the pigments up the chromatography paper through capillary action so that the pigments can be separated. The basic importance of chromatography is to separate different solvents by their molar mass. In doing so, one can separate the different pigments within a solution and measure how much of each pigment is present.
Why is chromatography used in the police?
the police use chromatography to solve crimes, for example if they wanted to solve if a drug or a substance was illegal or legal they would use paper chromatograhy which would mean they would place the substance or a drug on paper dip it in a testing liquid which could be water for example and if the paper turns purple for example that liquid may be illegal or if that dot of the certain substance rises over 5cm for example it may be illegal so chromatography comes in very handy for the police and solving there crimes ! Hope this helped ;) xxx
What is the purpose of double spotting the chromatography paper?
The chromatography paper is spotted twice so as to increase the amount of sample used for the separation on the paper. This is to allow distinct banding of the sample into its constituents.
Why must the boiling tube be stoppered by a cork in paper chromatography?
So that the harmful liquid does not come out and seriously injure you.
Uses for paper chromatography?
They are used in many scientific studies to identify unknown organic and inorganic compounds. They are also used in crime scene investigation, DNA and RNA sequencing, among others. Essentially, any solution can be separated through some form of chromatography.
What is the purpose of the solvent in chromatography?
The basic purpose of the solvent is to move the pigments up the chromatography paper through capillary action so that the pigments can be separated. The basic importance of chromatography is to separate different solvents by their molar mass. In doing so, one can separate the different pigments within a solution and measure how much of each pigment is present.
Why is chromatography used in the police?
the police use chromatography to solve crimes, for example if they wanted to solve if a drug or a substance was illegal or legal they would use paper chromatograhy which would mean they would place the substance or a drug on paper dip it in a testing liquid which could be water for example and if the paper turns purple for example that liquid may be illegal or if that dot of the certain substance rises over 5cm for example it may be illegal so chromatography comes in very handy for the police and solving there crimes ! Hope this helped ;) xxx
How can proteins be analyzed by chromatography?
A solution of amino acids is spotted onto a piece of chromatography paper which is then placed into a container filled with a suitable solvent. A dye is used so that the position of the amino acids along the piece of paper can be seen. The distances travelled by the amino acids are measured to calculate their retention factors (Rf) values. These are then compared to known standards.
Why are certain characters so interesting to us as readers?
Not all the readers find the same character interesting. The most easily they can relate to a character, the more they find it interesting.
Does each spot on the developed chromatography paper or plate represent one substance?
That is correct. Every chemical substance travels at its own speed when diffusing through paper, and so every substance will wind up at its own location on the paper or plate as the case may be.
Why did Fibonacci find his sequence so interesting?
Fibonacci found it interesting because he loved maths
What procedure did you use to separate plant pigments?
Place leaf in beaker and heat with Bunsen burner until water has discolored and boiled, then place leaf in test tube filled methylated spirits and place in same beaker as before with the burner turned OFF. Methylated spirits boils at around 80 degrees Celsius so when placed in heated water it will boil. This process will separate the chlorophyll (pigment) in the leaf.
How can paper chromatography be used to separate pigments based on their chemical and physical properties?
It is an appropriate technique to use because it separates the pigments, so one can see which pigments are present, even if some pigments are normally hidden to the naked eye.
Why ethanol not suitable in Gas chromatography?
Its alcohol so it can purify the chemicals used in the experiment. Furthermore, it will travel up the filter paper better than water will. Ethanol is pure whereas water may have other things in it such as chlorine is in tap water
