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Why do you need to cut the plasmid and cell DNA with the same restriction enzyme?
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What happens when a restriction enzyme is used on DNA?
when restriction enzyme is use on DNA basically it just first losen up the DNA, usally DNA is coiled, and so the restriction enzyme jsut breka the DNA and leave a sticky end, so that it can be put back together, the cell have to be able to do that because in nature, that's the way for cell to stop protein production and the cell still need that gene
What describes a plasmid?
a small organelle a small loop of DNA a prokaryotic ribosome a prokaryotic membrane
How many copies of one enzyme are in a cell?
Enzymes can have varying concentrations inside a cell. If the need for an enzyme is small then few enzymes will be in the cell however if there a signal to the cell that would cause a drastic need for more enzymes then the production and thus the concentration would increase.
What is one way to determine whether a bacterial culture has received a recombinant plasmid?
The plasmid have a "reporter gene" inside it, generally resistance to specific antibiotic. the plasmid is transformed into bacteria that don't have resistance to that specific antibiotic drug, and than the cultured on a petri-dish that contain the antibiotic drug. Only bacteria that had receive the plasmid will have resistance and grow, all the other will die.
What does the formation of recombinant DNA result from?
Recombinant DNATo to make recombinant DNA or plasmids, the two different samples of DNA need to be cut up by the same restriction enzyme. Restriction enzymes cut DNA at specific sequences (restriction sites) and is usually a staggered cut. For example, say you had the following sequence of DNA (both strands): 5' GAATTC 3'3' CTTAAG 5'Say the restriction enzyme used will cut a strand between a guanine and adenine on one strand and an adenine and guanine one the other strand. For the given DNA, there would be cuts where the bars are:5' G|AATTC 3'3' CTTAA|G 5'Then the strands would separate:5' G--------AATTC 3'3' CTTAA--------G 5'Because the cuts are staggered, hydrogen bonds are left open. The ends of the restriction fragments are called "sticky ends" because of their ability to bond to other fragments. Remember that both sets of DNA are cut with the same restriction enzyme. Therefore, the sticky ends of the restriction fragments are complementary to each other. Then you're able to take one fragment of one DNA sample and insert it into the other DNA sample, which are bound together by hydrogen bonds. DNA ligase is then added to seal the ends together.
What must researchers know before they begin the process of gentic engineering?
you need to know which restriction enzyme to use. also, who is the doner and the plasmid.
What happens when a restriction enzyme is used on DNA?
when restriction enzyme is use on DNA basically it just first losen up the DNA, usally DNA is coiled, and so the restriction enzyme jsut breka the DNA and leave a sticky end, so that it can be put back together, the cell have to be able to do that because in nature, that's the way for cell to stop protein production and the cell still need that gene
Why must you use an enzyme that will not cut anywhere within the gene that you are inserting into a plasmid?
If you are trying to take a gene from a DNA strand and put insert it into a plasmid, you wouldn't want a restriction enzyme to cut that gene up, or else it would be pretty useless. In other words, you need an enzyme or two that cuts outside that gene so that it can be functional after it's inserted into a plasmid. After your gene of interest is inserted into a plasmid, the plasmid can be put back into a bacterium, then you could genetically engineer plants with it or let the bacterium reproduce and produce many copies of a protein that you had wanted to make in the first place.
What describes a plasmid?
a small organelle a small loop of DNA a prokaryotic ribosome a prokaryotic membrane
How many copies of one enzyme are in a cell?
Enzymes can have varying concentrations inside a cell. If the need for an enzyme is small then few enzymes will be in the cell however if there a signal to the cell that would cause a drastic need for more enzymes then the production and thus the concentration would increase.
What is the role of BSA in restriction enzyme activity?
BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. This protein does not affect other enzymes that do not need it for stabilization.
What requires a host cell to reproduce?
yes a virus need host cell to repriduce becouse it lack enzyme system
If a cell needed to turn a polysaccharide into monosaccharides it would need to perform what?
It needs to hydrolyze (perform hydrolysis on) the polymer into monomers with an enzyme.
Need restriction code for Nokia 6300?
you can get restriction codes for your nokia mobile online sites that provides restriction codes.
Compare and contrast animanl and plant cells?
In bacteria, if the plasmid containing the foreign DNA manages to get inside a bacterial cell, this sequence ensures that it will be replicated. In Plant Cells, if transformation is successful the recombinant DNA is integrated into one of the chromosomes of the cell.
The takes up the plasmid. It now contains the human gene?
I think I know the answer... it's 5
Where is this enzyme produced?
You need to tell us which enzyme you're talking about.
