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Why determine motility and h2s before adding kovac's reagent?
You will do this regardless since Indole is part of the SIM media....this media will turn black if H2S is present and you read it for motility just like standard motility media. It must incubate for 24-28 hrs before doing the indole portion. Hope this helps.
What is a Kliger's iron agar and SIM have in common?
Kliger's Iron Agar (KIA) and Sulfur Indole Motility (SIM) can both test for the production of H2S, which binds with iron to form a black precipitate.
How is black pigment produced in the sim tube?
the organism inside the tube has produced hydrogen sulfide
What is the best biochemical test to identify gram negative and H2S bacilli?
Gram Stain to determine gram negative and shape or streak on a Agar plate selective for gram negative such as Desoxycholate Agar (DES). A SIM (Sulfur, Indole, Motility) Test or a Kiliger's Iron Agar (KIA) Test can confirm the production of H2S. *I would choose Streaking on DES to isolate the gram Negative bacteria, and then stab inoculate a SIM tube. Black precipitate confirms H2S production positive.
What is the component in the SIM deep tubes that makes this medium suitable to detect the production of indole?
The ingredients in SIM Medium enable the determination of three activities by which enteric bacteria can be differentiated. Sodium thiosulfate and ferrous ammonium sulfate are indicators of hydrogen sulfide production. The ferrous ammonium sulfate reacts with H2S gas to produce ferrous sulfide, a black precipitate.1 The casein peptone is rich in tryptophan, which is attacked by certain microorganisms resulting in the production of indole. The indole is detected by the addition of chemical reagents following the incubation period. Motility detection is possible due to the semisolid nature of the medium. Growth radiating out from the central stab line indicates that the test organism is motile.
What is a Kliger's iron agar and SIM have in common?
Kliger's Iron Agar (KIA) and Sulfur Indole Motility (SIM) can both test for the production of H2S, which binds with iron to form a black precipitate.
Why determine motility and h2s before adding kovac's reagent?
You will do this regardless since Indole is part of the SIM media....this media will turn black if H2S is present and you read it for motility just like standard motility media. It must incubate for 24-28 hrs before doing the indole portion. Hope this helps.
How is black pigment produced in the sim tube?
the organism inside the tube has produced hydrogen sulfide
Does proteus vulgaris produce h2s?
Yes, it does. It is Indole Production positive, H2S positive, and Motility positive. This can all be seen using SIM agar. Yes, it does. It is Indole Production positive, H2S positive, and Motility positive. This can all be seen using SIM agar.
What is the best biochemical test to identify gram negative and H2S bacilli?
Gram Stain to determine gram negative and shape or streak on a Agar plate selective for gram negative such as Desoxycholate Agar (DES). A SIM (Sulfur, Indole, Motility) Test or a Kiliger's Iron Agar (KIA) Test can confirm the production of H2S. *I would choose Streaking on DES to isolate the gram Negative bacteria, and then stab inoculate a SIM tube. Black precipitate confirms H2S production positive.
How do you turn a child sim into a plant sim?
turn him into a plant sim then cheat him to a kid.
Explain the function of the ferrous ammonium sulfate in SIM agar?
Its an indicator by combining with the gas forming an insoluble black ferrous sulfide precipitate that is seen along the line of the stab inoculation and is indicative of H2S production
What do you do when you are playing Sim's 2 for Xbox and the cheat trophy wont work?
Turn it off, and pop in Call of Duty: Black Ops.
How do you turn an alien sim back into normal in The Sims 2 PC game?
You can't turn an alien sim back to normal in the sims 2 for the pc. They really aren't different from regular sims except for that they have green skin and maybe even black eyes.
How do you turn into an alien on sim2 on ps2?
Your sims can not turn into an alien but u can create a sim as an alien by clicking on what skin color for your sim
How can you make your Sim turn into a werewolf?
A werewolve has to bite your sim but they have to become good friends.
How do you turn your sim into a werewolf on sims 2 pets?
You have your sim befriend the wolf pack leader (the wolf with glowing eyes), and then get it to bite your sim.
