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Q: Why does the negative control sample only produce one band?
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The OSOM card pregnancy test uses two bands to detect hCH the hormone of pregnancy, a (C) control band and a (T) test band. The test needs to be read three minutes after dropping urine in the sample well. If the C and T band is colored gray or black the test is positive for pregnancy (hCG is present). If only the C band is obvious the test is negative for detectable levels of hCG. If neither band is obvious the test is invalid, this could be due to age of the card or improper storage.


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SDS page resolve the proteins in the sample based on their molecular mass and the negative charge(and shape). if your sample has two proteins sure you can see two bands. if they are degraded by a unspecific protease during sample preparation it shows more than two bands. Same applies when you run "n" proteins, you can detect "n" number of bands.


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