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The short answer is residual calcium. Calcium mediates both transmitter release on the presynaptic side of the neuromuscular junction (where nerve meets muscle spindle) and calcium also mediates binding of the actin-myosin bridges that cause muscle contraction.

Here is a good source http://www.answers.com/neuromuscular+junction

The longer answer is much more interesting but takes some technical background. For a starters try a neurobiology textbook.

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Q: Why does the second contraction show higher than the first one on a monitor?
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