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Due to fluorescence, it absorbs UV and emits Orange light.. It is due to a phenyl group.. EtBr fluoresces even when not bound to DNA but its fluorescence increases 20 times when in bound state as hydrophobic environment between base pairs force dissociation of water bound to ethidium cation. Note: Water quenches fluorescence highly.

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Q: Why ethidium bromide gives orange color in UV light?
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