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Hydrochloric acid (HCl) is used in dilution for hemoglobin count because it helps to release hemoglobin from red blood cells, making it easier to measure accurately in laboratory tests. By breaking down the red blood cells, HCl facilitates the release of hemoglobin for a more precise quantification.

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1y ago

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Why is HCl used in sahlis method to determin hb count?

We add Hydrochloric acid (hcl) to convert hemoglobin to acid hematin which is a brownish red color, which we compare with the color of the glass of the Sahli apparatus (Hemoglobinometer). The meniscus (Glass containing the solution) should have marks thatmeasureboth the hemoglobin amount and percentage in the blood.


Why hcl is used in Hb estimation?

HCl (hydrochloric acid) is used in the estimation of hemoglobin (Hb) because it helps to release hemoglobin from red blood cells, making it more accessible for measurement. It also helps to remove other interfering substances, ensuring a more accurate estimation of hemoglobin levels.


How do you convert 0.1 N HCl to 0.01 N HCl?

To convert 0.1 N HCl to 0.01 N HCl, you can perform a dilution. For example, mix 10 mL of the 0.1 N HCl solution with 90 mL of distilled water to achieve a total volume of 100 mL, resulting in a 0.01 N HCl solution. The dilution factor here is 10, as you are reducing the concentration by a factor of ten.


What is Acid hematin Method?

it is formed when hcl reacts with hemoglobin ................it is the end product


How do you get 0.1M from 1M HCL?

To make a 0.1M solution from a 1M HCL solution, you would dilute the 1M HCL with 10 parts of water (or whatever solvent you are using). For example, mix 1 mL of 1M HCL with 9 mL of water to obtain a 0.1M HCL solution.


How do you turn 1N of HCL into 0.001N of HCL?

By dilution (1000x) with water: Take 1.0 mL 1.0N HCl and add up to 1000 mL with pure water.


How do you make 5mM HCl?

To make a 5mM solution of HCl, you would need to dilute concentrated HCl (usually in the range of 10-12M) with water. The exact dilution volume will depend on the concentration of the concentrated HCl solution you have. It's crucial to wear appropriate personal protective equipment and handle concentrated HCl with care due to its corrosive nature.


Why do you wait after adding the blood to hcl for ten minutes in sahli's method of hb?

Waiting for 10 minutes after adding blood to HCl in Sahli's method allows for complete lysis of red blood cells and release of hemoglobin. This step ensures that hemoglobin is in its free form and stable for accurate measurement.


How will you prepare one liter of 0.1 N HCL solution from 12 N HCL?

To prepare 1 liter of 0.1N HCl solution from 12N HCl, you would need to dilute the 12N HCl by a factor of 120. To do this, you would add approximately 83.33 mL of 12N HCl to a container and then dilute it with water to reach a final volume of 1 liter. Make sure to mix the solution thoroughly after dilution.


How many mL of conc. HCl equals 1 Liter 0.05 molar Hydrochloric acid?

To make 1 liter of 0.05 M HCl solution, you would need to dilute concentrated HCl. The concentration of the concentrated HCl would depend on its specific concentration, but you would typically need around 100 mL of concentrated HCl, assuming it is a standard 12 M concentration, to make the desired dilution.


How do you make 1M HCl from 12M HCl?

add water The above person is a troll.... This works for any volume of start and finish Litersc * Molesc / Litersc = Litersd * Molesd / Litersd where subscript c is concentrated and subscript d is dillute. The easier way to write this is C1V1=C2V2 so if you are going from 12M and want to go to 0.3M then it would be: 12M*V1=.3M*1L V1=.3ML/12M= 0.025L = 25mL


Is Ciproflocaxin hcl used to treat BV?

Ciproflocaxin hcl isn't used to treat BV.