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Which meniscus of acid hematin is considered for taking reading?
lower meniscus of acid hematin is considered while taking reading
Can dilute nitric or sulphuric acid be used instead of hydrochloric acid in sahlis acid hematin method?
Yes, diluted nitric or sulfuric acid can be used instead of hydrochloric acid in Sahli's acid hematin method. However, the choice of acid may affect the sensitivity and accuracy of the results, so adjustments may be needed in the protocol or interpretation of the test. It is advisable to validate the method with the alternative acid before routine use.
What is Acid hematin?
Acid hematin is a reddish-brown compound formed by the reaction of hemoglobin with acids. It is used in forensic science to detect the presence of bloodstains on fabrics or surfaces. By adding acid hematin solution to a suspected bloodstain, a characteristic green color change indicates the presence of blood.
Why is HCl used in sahlis method to determin hb count?
We add Hydrochloric acid (hcl) to convert hemoglobin to acid hematin which is a brownish red color, which we compare with the color of the glass of the Sahli apparatus (Hemoglobinometer). The meniscus (Glass containing the solution) should have marks thatmeasureboth the hemoglobin amount and percentage in the blood.
Why Hydrochloric acid is used for hemoglobin estimation?
to convert the Hb to acid-hematin which is a brown coloured complex.
What is the principle of hemoglobin estimation using cyanmethemoglobin method?
what are the various methods of the Hb estimation???/kindly tell me the senstivity of the methods. i know only one method sahli hemoglobinometer(acid hematin) method. I have enumerated few Sahli - Adam's Method Dare's method Haden's method Wintrobe's method Haldane's method Talliquist method Gasometric method Spectrophotometric method Automated & non-automated hemolglobinometry Other methods alkaline hematin method specific gravity comparator method
Why do we consider lower meniscus in acid hematin while taking a reading?
We consider the lower meniscus when reading acid hematin because the meniscus is the curved surface of a liquid, and the lower meniscus represents the bottom of the liquid column. This ensures that the measurement is accurate and consistent, as reading at the same point each time minimizes errors in volume determination.
Which meniscus of acid hematin is considered while taking a reading and why?
The upper meniscus of the acid hematin is considered when taking a reading because it represents the true volume of the sample. The meniscus is the curve at the surface of a liquid caused by surface tension, and in this case, the upper meniscus accounts for any excess liquid or bubbles that may be present on the surface.
What is assay method for formic acid?
Formic acid can be determined by ion chromatography.
What is the method of determining an acid and a base?
The pH scale
What is the laboratory method used to determine the concentration of an acid?
One common method to determine the concentration of an acid is titration. In an acid-base titration, a solution of known concentration (titrant) is added to the acid solution until the reaction is complete, as indicated by a color change with an indicator or a pH meter. The volume of titrant used is then used to calculate the concentration of the acid.
Why tartaric assay method falls under alkalimetric direct titration method?
The tartaric assay method falls under the alkalimetric direct titration method because it involves the direct titration of tartaric acid with a standardized alkali solution to determine its concentration. The method is based on the neutralization reaction between the tartaric acid and the alkali, which allows for the quantitative determination of the tartaric acid content present in the sample.
