Hemoglobin in GI tract converted to acid Hematin due to enzymatic digestion of hemoglobin; acid hematin is brown coffee color as opposed to bright red.
lower meniscus of acid hematin is considered while taking reading
Yes, diluted nitric or sulfuric acid can be used instead of hydrochloric acid in Sahli's acid hematin method. However, the choice of acid may affect the sensitivity and accuracy of the results, so adjustments may be needed in the protocol or interpretation of the test. It is advisable to validate the method with the alternative acid before routine use.
The upper meniscus of the acid hematin is considered when taking a reading because it represents the true volume of the sample. The meniscus is the curve at the surface of a liquid caused by surface tension, and in this case, the upper meniscus accounts for any excess liquid or bubbles that may be present on the surface.
We add Hydrochloric acid (hcl) to convert hemoglobin to acid hematin which is a brownish red color, which we compare with the color of the glass of the Sahli apparatus (Hemoglobinometer). The meniscus (Glass containing the solution) should have marks thatmeasureboth the hemoglobin amount and percentage in the blood.
# HydroFlouric acid # HydroCholoric acid # HydroBromic acid # Hydrogen Iodine # Carbonic acid # Hydronium acid # Sulferic acid # Nitric acid # Hydrogen Nitrate # Hydrogen Cyanide # Ribonucelic Acid # Deoxyribosenucleic Acid # Acetic Acid # Lactic Acid # Hydrogen Borate # Ascorbic Acir # Boraic Acid # AcetacyclicSalic Acid # Salic acid # Phosporic Acid
lower meniscus of acid hematin is considered while taking reading
to convert the Hb to acid-hematin which is a brown coloured complex.
it is formed when hcl reacts with hemoglobin ................it is the end product
Yes, diluted nitric or sulfuric acid can be used instead of hydrochloric acid in Sahli's acid hematin method. However, the choice of acid may affect the sensitivity and accuracy of the results, so adjustments may be needed in the protocol or interpretation of the test. It is advisable to validate the method with the alternative acid before routine use.
We consider the lower meniscus when reading acid hematin because the meniscus is the curved surface of a liquid, and the lower meniscus represents the bottom of the liquid column. This ensures that the measurement is accurate and consistent, as reading at the same point each time minimizes errors in volume determination.
The upper meniscus of the acid hematin is considered when taking a reading because it represents the true volume of the sample. The meniscus is the curve at the surface of a liquid caused by surface tension, and in this case, the upper meniscus accounts for any excess liquid or bubbles that may be present on the surface.
We add Hydrochloric acid (hcl) to convert hemoglobin to acid hematin which is a brownish red color, which we compare with the color of the glass of the Sahli apparatus (Hemoglobinometer). The meniscus (Glass containing the solution) should have marks thatmeasureboth the hemoglobin amount and percentage in the blood.
HCl (hydrochloric acid) is used in the estimation of hemoglobin (Hb) because it helps to release hemoglobin from red blood cells, making it more accessible for measurement. It also helps to remove other interfering substances, ensuring a more accurate estimation of hemoglobin levels.
what are the various methods of the Hb estimation???/kindly tell me the senstivity of the methods. i know only one method sahli hemoglobinometer(acid hematin) method. I have enumerated few Sahli - Adam's Method Dare's method Haden's method Wintrobe's method Haldane's method Talliquist method Gasometric method Spectrophotometric method Automated & non-automated hemolglobinometry Other methods alkaline hematin method specific gravity comparator method
Sulfuric acid, nitric acid, hydrochloric acid, phosphoric acid, hydrofluoric acid, formic acid, acetic acid, sulfurous acid, nitrous acid and hypochlorous acid are 10 acids.
# HydroFlouric acid # HydroCholoric acid # HydroBromic acid # Hydrogen Iodine # Carbonic acid # Hydronium acid # Sulferic acid # Nitric acid # Hydrogen Nitrate # Hydrogen Cyanide # Ribonucelic Acid # Deoxyribosenucleic Acid # Acetic Acid # Lactic Acid # Hydrogen Borate # Ascorbic Acir # Boraic Acid # AcetacyclicSalic Acid # Salic acid # Phosporic Acid
Sulfuric acid Hydrochloric acid Nitric acid