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Hemoglobin in GI tract converted to acid Hematin due to enzymatic digestion of hemoglobin; acid hematin is brown coffee color as opposed to bright red.
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∙ 13y ago
Acid hematin is a reddish-brown compound formed by the reaction of hemoglobin with acids. It is used in forensic science to detect the presence of bloodstains on fabrics or surfaces. By adding acid hematin solution to a suspected bloodstain, a characteristic green color change indicates the presence of blood.
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Which meniscus of acid hematin is considered for taking reading?
The upper meniscus of acid hematin is considered for taking reading in the acid hematin method to estimate hemoglobin concentration.
Can dilute nitric or sulphuric acid be used instead of hydrochloric acid in sahlis acid hematin method?
Yes, diluted nitric or sulfuric acid can be used instead of hydrochloric acid in Sahli's acid hematin method. However, the choice of acid may affect the sensitivity and accuracy of the results, so adjustments may be needed in the protocol or interpretation of the test. It is advisable to validate the method with the alternative acid before routine use.
Which meniscus of acid hematin is considered while taking a reading and why?
The upper meniscus of the acid hematin is considered when taking a reading because it represents the true volume of the sample. The meniscus is the curve at the surface of a liquid caused by surface tension, and in this case, the upper meniscus accounts for any excess liquid or bubbles that may be present on the surface.
Why is HCl used in sahlis method to determin hb count?
We add Hydrochloric acid (hcl) to convert hemoglobin to acid hematin which is a brownish red color, which we compare with the color of the glass of the Sahli apparatus (Hemoglobinometer). The meniscus (Glass containing the solution) should have marks thatmeasureboth the hemoglobin amount and percentage in the blood.
Give 20examples of acids?
Hydrochloric acid Sulfuric acid Nitric acid Acetic acid Citric acid Lactic acid Phosphoric acid Hydrofluoric acid Hydrobromic acid Formic acid Carbonic acid Hydroiodic acid Perchloric acid Tartaric acid Maleic acid Benzoic acid Propionic acid Butyric acid Oxalic acid Boric acid
Which meniscus of acid hematin is considered for taking reading?
The upper meniscus of acid hematin is considered for taking reading in the acid hematin method to estimate hemoglobin concentration.
Why Hydrochloric acid is used for hemoglobin estimation?
to convert the Hb to acid-hematin which is a brown coloured complex.
What is Acid hematin Method?
it is formed when hcl reacts with hemoglobin ................it is the end product
Can dilute nitric or sulphuric acid be used instead of hydrochloric acid in sahlis acid hematin method?
Yes, diluted nitric or sulfuric acid can be used instead of hydrochloric acid in Sahli's acid hematin method. However, the choice of acid may affect the sensitivity and accuracy of the results, so adjustments may be needed in the protocol or interpretation of the test. It is advisable to validate the method with the alternative acid before routine use.
Why do we consider lower meniscus in acid hematin while taking a reading?
We consider the lower meniscus when reading acid hematin because the meniscus is the curved surface of a liquid, and the lower meniscus represents the bottom of the liquid column. This ensures that the measurement is accurate and consistent, as reading at the same point each time minimizes errors in volume determination.
Which meniscus of acid hematin is considered while taking a reading and why?
The upper meniscus of the acid hematin is considered when taking a reading because it represents the true volume of the sample. The meniscus is the curve at the surface of a liquid caused by surface tension, and in this case, the upper meniscus accounts for any excess liquid or bubbles that may be present on the surface.
Why is HCl used in sahlis method to determin hb count?
We add Hydrochloric acid (hcl) to convert hemoglobin to acid hematin which is a brownish red color, which we compare with the color of the glass of the Sahli apparatus (Hemoglobinometer). The meniscus (Glass containing the solution) should have marks thatmeasureboth the hemoglobin amount and percentage in the blood.
Why hcl is used in Hb estimation?
HCl (hydrochloric acid) is used in the estimation of hemoglobin (Hb) because it helps to release hemoglobin from red blood cells, making it more accessible for measurement. It also helps to remove other interfering substances, ensuring a more accurate estimation of hemoglobin levels.
What is the principle of hemoglobin estimation using cyanmethemoglobin method?
what are the various methods of the Hb estimation???/kindly tell me the senstivity of the methods. i know only one method sahli hemoglobinometer(acid hematin) method. I have enumerated few Sahli - Adam's Method Dare's method Haden's method Wintrobe's method Haldane's method Talliquist method Gasometric method Spectrophotometric method Automated & non-automated hemolglobinometry Other methods alkaline hematin method specific gravity comparator method
Give 20examples of acids?
Hydrochloric acid Sulfuric acid Nitric acid Acetic acid Citric acid Lactic acid Phosphoric acid Hydrofluoric acid Hydrobromic acid Formic acid Carbonic acid Hydroiodic acid Perchloric acid Tartaric acid Maleic acid Benzoic acid Propionic acid Butyric acid Oxalic acid Boric acid
What are the names of 10 common acids?
Hydrochloric acid Sulfuric acid Nitric acid Acetic acid Citric acid Phosphoric acid Lactic acid Hydrofluoric acid Tartaric acid Carbonic acid
What are the four most important industrial acids?
Sulfuric acid, Hydrochloric acid, Nitric acid, Phosphoric Acid
