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Why does native DNA precipitate as fibers?
Native DNA precipitates as fibers when alcohol is added to a solution containing DNA. The DNA fibers can then be spooled onto a glass rod.
What is the percentage volume if 25 ml of ethyl alcohol is added to make 50 ml of solution?
50% by volume.
The concentration of alcohol and water solution is 25 percent alcohol by volume what is volume of alcohol and 200 lM of solution?
If the concentration of alcohol and water solution is 25 percent alcohol by volume, the volume of alcohol in a 200 solution is 50.
Role of phenol chloroform isoamyl alcohol in plasmid DNA extraction?
The actual role of phenol chloroform isoamyl alcohol in a plasmid DNA extraction is to purify the DNA. The alcohol will act in part as a detergent.
In a tank holding 30 gallons of solution 1 gallon is alcohol. What is the percentage of alcohol in the solution?
The percentage of alcohol is 3,33 %.
Why does native DNA precipitate as fibers?
Native DNA precipitates as fibers when alcohol is added to a solution containing DNA. The DNA fibers can then be spooled onto a glass rod.
Function of isoamyl alcohol in DNA extraction?
to separate two layer.DNA is insoluble in alcohol so it will floats up and all other cell components will precipitate at the bottom of the test tube.CIA also helps to isolate the CTAB solution so it will be easy to obtain the DNA.
What is the function in the following reagents used in the extraction and precipitation of DNA?
to extract the DNA from the solution, isopropyl alcohol is mixed with the DNA solution to precipitate the same.
Why does the DNA rise into the layer of alcohol?
When in solution, DNA is surrounded by a shell of hydration (or ajacket of water molecules). Ethanol is a dehydrating agent. Upon addition of ethanol, water molecules get sequestered and the DNA comes out of solution, a phenomenon commonly refered to as DNA precipitation. Precipitated DNA can be seen with the naked eye
What is the function of the alcohol in DNA extraction?
DNA is not soluble in isopropyl alcohol. It will precipitate out when you add this solvent. Once out of solution you can centrifuge it down and collect the pellet of DNA.
What is the purpose of the alcohol in the strawberry DNA extraction lab?
Hmm, I think it's used to wash the DNA from the substance used for extracting the DNA, such as Isopropanol, CLS, NLS, PPS which are used in the process of extraction to the process to precipitate the DNA (using isopropanol). DNA is insoluable in alcohol. Actually the real reason for using alcohol in a DNA extraction is to "precipitate" (NOT RAIN) the DNA, as you may have realized when you did the experiment , the Alcohol step is the last step that brings the DNA to the surface. Finally alcohol can be added so that the DNA can be seen, but first it is crucial to know why the addition of alcohol is such an important step. This step is closely related to a process called precipitation, which is " the process of separating a substance from a solution as a solid" (answers.com, precipitation). Precipitation separates the DNA so that it can no longer remain dissolved and so that it can be seen. DNA is less dense than water and alcohol , so the DNA rises to the alcohol layer. Also the DNA is soluble in water and insoluble in alcohol, so when it is just in the mixture it is quite invisible but once the alcohol is added it condensates by precipitation to the point where it is very visible.
How much water must be added to 12 L of a 40 percent solution of alcohol to obtain a 30 percent solution?
4 litres
Why is isopropanol used to precipitate DNA and what does it do to DNA to make it precipitate?
As DNA is completely soluble in water, but not in alcohol, like isopropanol, when isoprop is added, its engaged more and more water molecule to interact, as a result, less water molecules are available to dissolve DNA, and DNA statrs ppt out.
Why is detergent added to the extraction buffer?
The detergent was added because it breaks open the cells by destroying fatty membranes that enclose the cell, thus releasing the DNA into the solution.
What is the percentage volume if 25 ml of ethyl alcohol is added to make 50 ml of solution?
50% by volume.
The concentration of alcohol and water solution is 25 percent alcohol by volume what is volume of alcohol and 200 lM of solution?
If the concentration of alcohol and water solution is 25 percent alcohol by volume, the volume of alcohol in a 200 solution is 50.
How many ml of water should be added to 95 percent ethyl alcohol to make 1 liter of a 30 percent ethyl alcohol solution?
684 ml
