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In pour plate technique the culture to be grwon is pour in melted agar medium, now when we add the diluted sample in agar plate and if the melted agar is very hot, it can lead to the damage of bacterial or fungal cell and may cause in abruption of growth, so the agar is cooled to get the optimum temp. for growth of microbial cell.

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13y ago
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14y ago

if im right the culture medium is cooled so as not to destroy any sensitive bacteria.

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13y ago

The culture medium is cooled so that it does not melt the plastic petri dish.

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Q: Why is culture medium cooled 48 temperature before is poured?
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